foreign activity
RNase, none detected
storage temp.
−20°C
Application
For polyadenylated mRNA purification using rapid and simple chromatographic techniques. Covalently attached oligothymidylic acid chains are approx. 20 nucleotides long. Eukaryotic mRNA specifically binds by its 3′-poly(A) tail. Non-polyadenylated RNA and other contaminants can be removed by washing, and the purified mRNA is eluted with a low salt buffer.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
相关内容
Datasheet
Nikolay E Shirokikh et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(31), 10738-10743 (2008-07-29)
Eukaryotic mRNAs in which a poly(A) sequence precedes the initiation codon are known to exhibit a significantly enhanced cap-independent translation, both in vivo and in cell-free translation systems. Consistent with high expression levels of poxviral mRNAs, they contain poly(A) sequences
Gilham
Journal of the American Chemical Society, 86, 4982-4982 (1964)
H Aviv et al.
Proceedings of the National Academy of Sciences of the United States of America, 69(6), 1408-1412 (1972-06-01)
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active