OGS140
PSF-CMV-COOH-EKT-GST3 - C-TERMINAL GST PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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NACRES:
NA.85
UNSPSC Code:
12352200
Promoter:
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
Promoter activity: constitutive
Promoter type: mammalian
Origin of replication:
pUC (500 copies)
Bacteria selection:
kanamycin
Reporter gene:
none
Peptide cleavage:
EKT
tag
GST tagged
form
buffered aqueous solution
mol wt
size 4900 bp
bacteria selection
kanamycin
origin of replication
pUC (500 copies)
peptide cleavage
EKT
peptide tag location
C-terminal
promoter
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
reporter gene
none
shipped in
ambient
storage temp.
−20°C
General description
This vector adds a GST tag positioned at the 3 prime end (C-terminus) of a gene (expressed protein) that is within the primary MCS (NotI to XbaI). The tag can be used to purify a protein by binding to glutathione and the tag can also be used to increase the solubility of recombinant protein produced in bacterial cells. For reference the coding sequence of the GST tag is one base pair out of frame with the TAG stop codon that is found within the upstream XbaI site. There is an enterokinase cleavage site immediately upstream of this tag (DDDDK) that can be used to remove the tag from a purified protein if required.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Application
Cloning in a gene: There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI. There are built in Shine-Dalgarno and KOZAK sequences aligned with the start codon within the NcoI restriction site. If these are removed you will need to add a ribosomal entry site to the 5 prime end of your gene for optimal expression.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.
Other Notes
Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Legal Information
Oxford Genetics is a trademark of Oxford Genetics Ltd
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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| 货号 | GTIN |
|---|---|
| OGS140-5UG | 04061837165337 |
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