OGS2806
PSF-OXB20-NH2-10HIS-EKT - N-TERMINAL 10HIS TAG BACTERIAL PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
产品名称
PSF-OXB20-NH2-10HIS-EKT - N-TERMINAL 10HIS TAG BACTERIAL PLASMID, plasmid vector for molecular cloning
recombinant
expressed in E. coli
tag
10-His tagged
form
buffered aqueous solution
mol wt
size 4982 bp
bacteria selection
kanamycin
origin of replication
pUC (500 copies)
peptide cleavage
EKT
peptide tag location
N-terminal
promoter
Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial
reporter gene
none
shipped in
ambient
storage temp.
−20°C
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxgene.com
General description
This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.
About the Peptide Tag:This plasmid contains an n-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.
Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
About the Peptide Tag:This plasmid contains an n-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.
Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
Other Notes
To view sequence information for this product, please visit the product page
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..
Plasmid platform with interchangeable DNA components offers versatile research tools for genetic studies.
Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research.
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