OGS3099
PSF-OXB20-NH2-OMPA - OMPA SECRETION PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
重组
expressed in E. coli
表单
buffered aqueous solution
分子量
size 3911 bp
菌种筛选
kanamycin
复制起点
pUC (500 copies)
肽切割
no cleavage
肽标签位置
N-terminal
启动子
Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial
报告基因
none
分泌信号
OmpA
运输
ambient
储存温度
−20°C
一般描述
This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.
About the Peptide Tag:This plasmid contains an Outer Membrane Protein A (OmpA) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: PSF-OXB20-NH2-OMPA - OMPA secretion plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
OXB20-NH2-OMPA - OMPA secretion plasmid is an N-terminal OmpA secretory signal peptide plasmid. This bacterial expression vector has no protease or other tags.
About the Peptide Tag:This plasmid contains an Outer Membrane Protein A (OmpA) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: PSF-OXB20-NH2-OMPA - OMPA secretion plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
OXB20-NH2-OMPA - OMPA secretion plasmid is an N-terminal OmpA secretory signal peptide plasmid. This bacterial expression vector has no protease or other tags.
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
其他说明
To view sequence information for this product, please visit the product page
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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商品
SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..
Plasmid platform with interchangeable DNA components offers versatile research tools for genetic studies.
Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research.
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