P0520
磷酸二酯酶 3′,5′-环核苷酸（活化剂不足） 来源于牛心脏
lyophilized powder (contains imidazole buffer salts and magnesium sulfate), >0.1 units/mg protein (with added activator), PI fraction of Ho, H.C., et al., Biochim. Biophys. Acta, 429, 461 (1976).
别名:
3′,5′-环核苷酸 5′-核苷酸水解酶 来源于牛心脏, PDE
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关于此项目
Specific activity:
>0.1 units/mg protein (with added activator)
Biological source:
bovine heart
biological source
bovine heart
form
lyophilized powder (contains imidazole buffer salts and magnesium sulfate)
specific activity
>0.1 units/mg protein (with added activator)
mol wt
~60 kDa
composition
Protein, ~75%
UniProt accession no.
foreign activity
5′-ATPase, alkaline phosphatase, and 2′:3′-cyclic nucleotide phosphodiesterase <0.005 units/mg protein, 5′-nucleotidase <0.01 units/mg protein, inorganic pyrophosphatase <0.5 units/mg protein
storage temp.
−20°C
Gene Information
cow ... PDE1A(281969)
Application
Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca2+. It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold.
For use in calmodulin (P 0270) assay. Activity is reduced to <50% without calmodulin present.
Biochem/physiol Actions
水解环单磷酸核苷酸（如cAMP 和 cGMP）的 3′,5′-磷酸二酯键，生成相应的 5′-单磷酸核苷酸。
The herbicide Atrazine was shown inhibit phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart.
Packaging
根据活化单位确定包装尺寸
Preparation Note
已除去蛋白质活化剂钙调蛋白的天然制剂。
用 50% 甘油复原。在 -0°C 下储存，酶的总活化单位可保持至少 5 天的恒定状态。但是，活化剂不足时的活性可增加至 200%。如果在 4°C 下储存，激活时的活性和活化剂不足时的活性都将在 24 小时内下降约 30%。
Analysis Note
除非特别说明，否则均采用 Warburg-Christian 法测定蛋白质。
Other Notes
在 pH 7.5 和 30°C 下，一单位本品每分钟可将 1.0μmol 3′:5′-cAMP 水解为 5′-AMP。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Mark Roberge et al.
Toxicology letters, 154(1-2), 61-68 (2004-10-12)
Atrazine (ATR), 2-chloro-4-ethylamino-6-isopropylamino-s-triazine, has been implicated in numerous studies to act as an endocrine disruptor, specifically by altering estradiol signaling via increased aromatase activity. Fluorescence polarization (FP) was used to show that the binding equilibria between estrogen receptor-alpha or estrogen
C Y Huang et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(2), 871-874 (1981-02-01)
Kinetic studies on the activation of cyclic nucleotide phosphodiesterase (3',5'-cyclic-nucleotide 5'-nucleotidohydrolase, EC 3.1.4.17) as a function of calmodulin and Ca2+ concentrations have been carried out. A general approach to analyzing the mechanism of activation, which takes into consideration the various
Binding of trifluoperazine to the calcium-dependent activator of cyclic nucleotide phosphodiesterase.
R M Levin et al.
Molecular pharmacology, 13(4), 690-697 (1977-07-01)
T Kaneko et al.
Cellular signalling, 7(5), 527-534 (1995-07-01)
Elevated intracellular cyclic AMP is associated with the inhibition of many inflammatory cellular responses. In this study, we examined the effect of cyclic AMP on eosinophil chemotaxis. Eosinophils were isolated from healthy human volunteers using an immunomagnetic method. Eosinophils were
Evidence that calmodulin may be involved in phytohaemagglutinin-stimulated lymphocyte division.
S Mac Neil et al.
Bioscience reports, 2(11), 891-897 (1982-11-01)
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