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Merck
CN

P1609

Sigma-Aldrich

Protein Kinase C Catalytic Subunit from rat brain

≥90% (SDS-PAGE), ≥800 units/mg protein

别名:

PKC-M, PKM

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关于此项目

MDL编号:
UNSPSC代码:
12352204
eCl@ss:
32160410
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方案

≥90% (SDS-PAGE)

表单

liquid

比活

≥800 units/mg protein

包装

vial of 200 ng

浓度

25 μg/mL protein

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

rat ... Prkd1(85421)

生化/生理作用

Does not require Ca2+ or phosphatidylserine for its activity.

外形

Storage buffer: 20 mM Tris-HCl, pH 7.5, 2 mM EGTA, 2 mM EDTA, 1 mM DTT, 10 mM potassium phosphate, 50% glycerol, 0.05% Triton X-100

制备说明

Prepared by tryptic digestion of PKC.

其他说明

One unit will transfer of 1 nanomole of phosphate per minute at 30 °C using Histone III as substrate and phosphatidylserine as an activator.

法律信息

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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B VanRenterghem et al.
The Journal of biological chemistry, 269(40), 24666-24672 (1994-10-07)
Previously pp60v-src, cyclin A, p39mos, and maturation-promoting factor (composed of Cdc2 and cyclin B) have been shown to activate mitogen-activated protein kinase (MAPK) and MAPK kinase (MEK) in cell-free extracts of Xenopus oocytes. The pp60v-src pathway is dependent on a
K P Huang et al.
Biochemical and biophysical research communications, 139(1), 320-326 (1986-08-29)
Tryptic fragments of protein kinase C containing the kinase (45 KDa) and phorbol ester-binding activity (38 KDa) were separated by Mono O column chromatography. The purified phorbol ester-binding fragment exhibits a higher affinity for phosphatidylserine than the native enzyme but

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