P1790
Phosphodiesterase, 3′,5′-Cyclic Nucleotide, Activator-deficient from porcine brain
lyophilized powder
别名:
3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from porcine brain, PDE
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关于此项目
form
lyophilized powder
specific activity
1.0-5.0 units/mg protein (without added calmodulin.)
mol wt
~60 kDa
purified by
affinity chromatography
contains
lactose as stabilizer
composition
Protein, ~5% Lowry
UniProt accession no.
foreign activity
5′-ATPase, inorganic pyrophosphatase, alkaline phosphatase and 2′,3′-cyclic nucleotide phosphodiesterase <0.1%, 5′-nucleotidase <0.5%
storage temp.
−20°C
Gene Information
pig ... PDE3A(396555)
Application
May be used to assay the protein activator, calmodulin.
Biochem/physiol Actions
Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.
Packaging
Package size based on activated units.
Physical form
Lyophilized preparation which has been depleted of calmodulin and containing buffer salts as Tris-HCl.
Preparation Note
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approx. 30% in 24 hrs. if stored at 4 °C.
Analysis Note
Protein determined by Warburg-Christian unless specified otherwise.
Other Notes
One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Chimed Jansen et al.
Journal of medicinal chemistry, 56(5), 2087-2096 (2013-02-16)
Trypanosoma brucei cyclic nucleotide phosphodiesterase B1 (TbrPDEB1) and TbrPDEB2 have recently been validated as new therapeutic targets for human African trypanosomiasis by both genetic and pharmacological means. In this study we report the crystal structure of the catalytic domain of
N B Matveeva et al.
Biofizika, 57(5), 832-839 (2012-11-10)
Possible involvement of autocrine factors into the control of motile behavior via a receptor-mediated mechanism was investigated in Physarum polycephalum plasmodium, a multinuclear amoeboid cell with the auto-oscillatory mode of motility. Cyclic adenosine monophosphate (cAMP) and extracellular cAMP-specific phosphodiesterase, its
Tamam El-Elimat et al.
Journal of natural products, 76(3), 382-387 (2013-01-11)
Three bioactive compounds were isolated from an organic extract of an ascomycete fungus of the order Chaetothyriales (MSX 47445) using bioactivity-directed fractionation as part of a search for anticancer leads from filamentous fungi. Of these, two were benzoquinones [betulinan A