P1790
Phosphodiesterase, 3′,5′-Cyclic Nucleotide, Activator-deficient from porcine brain
lyophilized powder
别名:
3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from porcine brain, PDE
form
lyophilized powder
specific activity
1.0-5.0 units/mg protein (without added calmodulin.)
mol wt
~60 kDa
purified by
affinity chromatography
contains
lactose as stabilizer
composition
Protein, ~5% Lowry
UniProt accession no.
foreign activity
5′-ATPase, inorganic pyrophosphatase, alkaline phosphatase and 2′,3′-cyclic nucleotide phosphodiesterase <0.1%, 5′-nucleotidase <0.5%
storage temp.
−20°C
Gene Information
pig ... PDE3A(396555)
Application
May be used to assay the protein activator, calmodulin.
Biochem/physiol Actions
Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.
Packaging
Package size based on activated units.
Physical form
Lyophilized preparation which has been depleted of calmodulin and containing buffer salts as Tris-HCl.
Preparation Note
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approx. 30% in 24 hrs. if stored at 4 °C.
Analysis Note
Protein determined by Warburg-Christian unless specified otherwise.
Other Notes
One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
The cyclic AMP pathway.
Paolo Sassone-Corsi
Cold Spring Harbor perspectives in biology, 4(12), doi:10-doi:10 (2012-12-05)
Ryan T Cameron et al.
Biochemical pharmacology, 85(9), 1297-1305 (2013-03-12)
PDE4 is one of eleven known cyclic nucleotide phosphodiesterase families and plays a pivotal role in mediating hydrolytic degradation of the important cyclic nucleotide second messenger, cyclic 3'5' adenosine monophosphate (cAMP). PDE4 inhibitors are known to have anti-inflammatory properties, but
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Brown adipose tissue (BAT) is a highly thermogenic organ that converts lipids and glucose into heat. Many of the metabolic and gene transcriptional hallmarks of BAT activation, namely increased lipolysis, uncoupling protein-1 (UCP1) mRNA, and glucose uptake, are regulated by
One single signaling pathway for so many different biological functions: lessons from the cyclic adenosine monophosphate/protein kinase A pathway-related diseases.
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The Journal of clinical endocrinology and metabolism, 97(12), 4355-4357 (2012-12-12)
Koji Ochiai et al.
Bioorganic & medicinal chemistry letters, 23(1), 375-381 (2012-12-04)
(-)-6-(7-Methoxy-2-(trifluoromethyl)pyrazolo[1,5-a]pyridin-4-yl)-5-methyl-4,5-dihydropyridazin-3(2H)-one (KCA-1490) exhibits moderate dual PDE3/4-inhibitory activity and promises as a combined bronchodilatory/anti-inflammatory agent. N-alkylation of the pyridazinone ring markedly enhances potency against PDE4 but suppresses PDE3 inhibition. Addition of a 6-aryl-4,5-dihydropyridazin-3(2H)-one extension to the N-alkyl group facilitates both enhancement
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