Readily reproducible and extensively annotated, methods covered include culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants.

Part I. Introduction. An Introduction to Plant Cell Culture: Pointers to Success, Part II. Cell Culture and Plant Regeneration. Callus Initiation, Maintenance, and Shoot Induction in Rice, Callus Initiation, Maintenance, and Shoot Induction in Potato, Somatic Embryogenesis in Barley Suspension Cultures. Somatic Embryogenesis in Picea Suspension Cultures. Specialized Techniques. Direct, Cyclic Somatic Embryogenesis in Cassava for Mass Production Purposes, Immature Inflorescence Culture of Cereals. Cryopreservation of Rice Tissue Cultures. Noncryogenic Long-Term Germplasm Storage. Part III. Plant Propagation In Vitro. Microprogation of Strawberry via Axillary Shoot Proliferation. Meristem-Tip Culture for Propagation and Virus Elimination. Specialized Techniques. Clonal Propagation of Orchids, In Vitro Propagation of Succulent Plants. Microproragation of Flower Bulbs: Lily and Narcissus. Clonal Propagation of Woody Species. Spore-Derived Axenic Cultures of Ferns as a Method of Propagation. Part IV. Applications for Plant Protoplasts. Protoplast Isolation, Culture, and Plant Regeneration from Passiflora Leaves. Isolation, Culture, and Plant Regeneration from Suspension-Derived Protoplasts of Lolium. Protoplast Fusion for Symmetric Somatic Hybrid Production in Brassicaceae, J. Production of Cybrids in Rapeseed (Brassica napus), Specialized Techniques. Microprotoplast-Mediated Chromosome Transfer (MMCT) for the Direct Production of Monosomic Addition Lines. Guard Cell Protoplasts: Isolation, Culture, and Regeneration of Plants. In Vitro Fertilization with Isolated, Single Gametes, Part V. Protocols for Genomic Manipulation. Protocols for Anther and Microspore Culture of Barley, Microspore Embryogenesis and In Vitro Pollen Maturation in Tobacco. Embryo Rescue Following Wide Crosses. Specialized Techniques. Mutagenesis and the Selection of Resistant Mutants, The Generation of Plastid Mutants In Vitro, Part VI. Protocols for the Introduction of Specific Genes. Agrobacterium-Mediated Transformation of Petunia Leaf Discs. Transformation of Rice Via PEG-Mediated DNA Uptake into Protoplasts. Transformation of Wheat Via Particle Bombardment, Plant Transformation Via Protoplast Electroporation, Specialized Techniques. Transformation of Maize Via Tissue Electroporation, Transformation of Maize Using Silicon Carbide Whiskers. Part VII. Suspension Culture Initiation and the Accumulation of Metabolites. Directing Anthraquinone Accumulation Via Manipulation of Morinda Suspension Cultures. Alkaloid Accumulation in Catharanthus Roseus Suspension Cultures, Betalains: Their Accumulation and Release In Vitro. Appendix. Index.