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Merck
CN

P2973

Sigma-Aldrich

(−21) M13正向引物组

别名:

5′-GTA AAA CGA CGG CCA GT-3′

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UNSPSC代码:
41106305
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储存温度

2-8°C

应用

经过功能测试,可用于荧光检测自动测序。
(-21)M13正向引物组包含具有5′-羟基和3′-羟基末端的单链寡核苷酸,以及为聚合酶链反应实验方案(PCR)所选择的四种荧光标记。

包装

采用琥珀色管包装,防止光线照射。

分析说明

纯度已通过HPLC、PAGE和OD进行测试。

其他说明

一组四瓶引物,每个引物在5′末端单独标记有:
FAM:(5 [6]-羧基荧光素)
JOE:( 6-羧基-4′,5′-二氯-2′,7′-二甲氧基荧光素)
ROX:(5 [6]-羧基-X-罗丹明),以及
TAMRA:(5 [6]-羧基四甲基罗丹明)。
以溶于tris-EDTA缓冲液(pH 7.5)的溶液形式提供,每瓶50 μl,溶度1pmole/ μl

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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High-throughput sequencing of PCR products tagged with universal primers using 454 life sciences systems.
Daigle D, Simen BB, Pochart P.
Current Protocols in Molecular Biology, Unit7-Unit7 (2011)
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PloS one, 6(7), e22224-e22224 (2011-07-27)
Eukaryotic DNA polymerase δ (pol δ) plays a crucial role in chromosomal DNA replication and various DNA repair processes. It is thought to consist of p125, p66 (p68), p50 and p12 subunits. However, rigorous isolation of mammalian pol δ from
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Genetics and molecular research : GMR, 9(4), 2016-2024 (2010-10-20)
Seven fungal isolates were identified as pan-global Hypocrea/Trichoderma species, from section Trichoderma, on the basis of their morphology. These species were H. lixii/T. harzianum and H. orientalis/T. longibrachiatum. PCR-based markers with primer M13 (core sequence of phage M13) and internal-transcribed
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International journal of food microbiology, 144(1), 187-192 (2010-10-12)
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