生物来源
rabbit
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous glycerol solution
分子量
antigen 65 kDa
种属反应性
rat, mouse
技术
immunoprecipitation (IP): 4 μg using 0.5 mg of mouse brain cytosol
western blot (chemiluminescent): 2 μg/mL using mouse 3T3 fibroblasts extract or mouse brain extract
UniProt登记号
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
mouse ... Pak3(18481)
rat ... Pak3(29433)
一般描述
p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
Anti-PAK3 specifically reacts with mouse and rat PAK3 (65 kDa). It reacts weakly with human PAK3.
Anti-PAK3 specifically reacts with mouse and rat PAK3 (65 kDa). It reacts weakly with human PAK3.
免疫原
synthetic peptide corresponding to amino acids 1-13 of the N-terminal of mouse p21 activated kinase 3 (PAK3).
应用
Anti-PAK3 may be used at a concentration of 4 μg to immunoprecipitate PAK3 from 0.5-1 mg mouse NIH3T3 total cell lysate. It may be used at a concentration of 1-2 μg/mL for detection in total mouse NIH3T3 lysate by immunoblotting.
外形
Solution in 0.07 M Tris-glycine, pH 7.4, containing 30% glycerol and 0.035% sodium azide.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
新产品
此项目有
The genetics of Pak
Hofmann C et al
Journal of Cell Science, 17, 4343-4354 (2004)
Zhou-shen Zhao et al.
The Biochemical journal, 386(Pt 2), 201-214 (2004-11-19)
The Rho GTPases are a family of molecular switches that are critical regulators of signal transduction pathways in eukaryotic cells. They are known principally for their role in regulating the cytoskeleton, and do so by recruiting a variety of downstream
Mengwei Zang et al.
The Journal of biological chemistry, 277(6), 4395-4405 (2001-12-26)
Activation of Raf-1 is a complex process in which phosphorylation of Ser(338)-Tyr(341) is a critical step. Previous studies have shown that Pak1/2 is implicated in both Ras-dependent and -independent activation of Raf-1 by phosphorylating Raf Ser(338). The present study explores
Christy C Ong et al.
Oncotarget, 2(6), 491-496 (2011-06-10)
The p21-activated kinases (PAKs) are central players in growth factor signaling networks and morphogenetic processes that control proliferation, cell polarity, invasion and actin cytoskeleton organization. This raises the possibility that interfering with PAK activity may produce significant anti-tumor activity. In
C Chong et al.
The Journal of biological chemistry, 276(20), 17347-17353 (2001-03-30)
The p21-activated kinases (PAKs), in common with many kinases, undergo multiple autophosphorylation events upon interaction with appropriate activators. The Cdc42-induced phosphorylation of PAK serves in part to dissociate the kinase from its partners PIX and Nck. Here we investigate in
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