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Merck
CN

P2992

Sigma-Aldrich

Monoclonal Anti-p59fyn antibody produced in mouse

~1 mg/mL, clone 1S, purified immunoglobulin, buffered aqueous solution

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MDL编号:
UNSPSC代码:
12352203
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生物来源

mouse

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

1S, monoclonal

表单

buffered aqueous solution

种属反应性

human, rat, mouse

浓度

~1 mg/mL

技术

capture ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-5 μg/mL using human lymphoma tissue
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG1

UniProt登记号

运输

wet ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... FYN(2534)
mouse ... Fyn(14360)
rat ... Fyn(25150)

一般描述

The signalling in T cells is mediated by receptor and non-receptor protein tyrosine kinases (PTK). Two non-receptors PTK that belong to Src family, Fyn and Lck are vital for T-cell development and function. Both Fyn and Lck have unique domains that undergo post-translational modifications such as palmitoylation, myristoylation and acetylation od specific cysteine residues that are critical for T cell signalling. Fyn is generally associated with intracellular structures such as mitotic spindles. Both Fyn and Lck are closely associated in T cell differentiation, expansion and mediating TCR-mediated survival signals in naïve T cells. Both the kinases have partially overlapping functions and have the ability to compensate if either kinase is deficient.
Monoclonal Anti- p59fyn specifically recognizes human, mouse and rat Fyn protein (~59 kDa).

免疫原

amino acids 1-206 from recombinant murine p59fyn

应用

Monoclonal Anti- p59fyn may be used for detection by immunohistochemistry of formalin-fixed, paraffin-embedded human lymphoid tissue sections at a working concentration of 1-5 μg/mL. The recommended concentration for immunoprecipitation is 2μg/mg of lysate. The antibody is also suitable for immunoblotting and ELISA.

外形

Solution in phosphate buffered saline pH 7.4, containing 0.08% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Dominik Filipp et al.
Molecular immunology, 41(6-7), 645-656 (2004-06-29)
While both lck and fyn are essential to generating the full sequelae of proximal signals emanating from the TcR/CD3 complex, thus far, modeling the temporal and spatial involvement of fyn has been problematic. The absence of a binding partner, analogous
A Wolven et al.
Molecular biology of the cell, 8(6), 1159-1173 (1997-06-01)
Members of the Src family of protein tyrosine kinases are localized to subspecialized regions of the plasma membrane. Herein we show that the N-terminal SH4 region of the Src family member p59fyn (Fyn) is both necessary and sufficient for targeting
M Koegl et al.
The Biochemical journal, 303 ( Pt 3), 749-753 (1994-11-01)
We have recently identified a novel N-terminal cysteine-containing motif which specifies the palmitoylation of several G-protein alpha-subunits [Parenti, Viganó, Newman, Milligan and Magee (1993) Biochem. J. 291, 349-353]. A related motif occurs at the N-terminus of members of the Src
S C Ley et al.
The Journal of cell biology, 125(3), 639-649 (1994-05-01)
Two src family kinases, lck and fyn, participate in the activation of T lymphocytes. Both of these protein tyrosine kinases are thought to function via their interaction with cell surface receptors. Thus, lck is associated with CD4, CD8, and Thy-1
Dominik Filipp et al.
The Journal of biological chemistry, 283(39), 26409-26422 (2008-07-29)
Mechanisms regulating the activation and delivery of function of Lck and Fyn are central to the generation of the most proximal signaling events emanating from the T cell antigen receptor (TcR) complex. Recent results demonstrate that lipid rafts (LR) segregate

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