P3752
酸性磷酸酶 来源于马铃薯
lyophilized powder, ≥0.5 unit/mg solid
别名:
(Orthophosphoric-monoester phosphohydrolase acid optimum), 酸性磷酸酶 来源于马铃薯
应用
酸性磷酸酶通常用于蛋白质和核酸的去磷酸化。 酸性磷酸酶是一种酸性水解酶,通常存在于溶酶体中。它可作为鉴定亚细胞组分中溶酶体的标记物。 酸性磷酸酶(产品P3752)已被作为对照,用于研究 牛分枝杆菌 BCG如何影响肺泡巨噬细胞中酸性磷酸酶的活性水平。
生化/生理作用
酸性磷酸酶是一种细胞质溶酶体水解酶,可催化正磷酸单酯和水转化为醇和正磷酸盐。
其他说明
在pH 4.8,37 °C条件下,一个单位可在每分钟水解1.0 μmole 对硝基苯磷酸盐。
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Filipe Natalio et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 23(21), 4973-4980 (2017-03-02)
Boron's unusual properties inspired major advances in chemistry. In nature, the existence and importance of boron has been fairly explored (e.g. bacterial signaling, plant development) but its role as biological catalyst was never reported. Here, we show that boric acid
I N Mbawuike et al.
Infection and immunity, 51(2), 483-489 (1986-02-01)
The kinetics of induction of the bronchoalveolar cell population (i.e., alveolar macrophages [AM], lymphocytes, and polymorphonuclear leukocytes) was studied in mice inoculated intravenously with heat-killed Mycobacterium bovis BCG. Injection of BCG at 100 and 500 micrograms but not at 10
K Zyła et al.
The British journal of nutrition, 74(1), 3-17 (1995-07-01)
An in vitro method was developed to predict inorganic P release from maize-soyabean poultry feeds containing supplemental phytase (EC 3.1.3.8), and to quantify the effect of acid phosphatase (EC 3.1.3.2), fungal protease (EC 3.4.23.6) and Aspergillus niger cellulase (EC 3.2.1.4)
Jia Zhang et al.
Biosensors & bioelectronics, 141, 111442-111442 (2019-06-30)
Phenol contains an exchangeable hydroxyl proton resonant at 4.8 ppm from the resonance frequency of water in the 1H nuclear magnetic resonance (1H NMR) spectrum, enabling itself to be detected at sub-mM concentration by either chemical exchange saturation transfer magnetic resonance
Feliznando Isidro Cárdenas-Torres et al.
Medicina (Kaunas, Lithuania), 55(3) (2019-03-23)
Background: The first cases of food allergy to amaranth grain have recently been published. This pseudocereal is considered hypoallergenic, and there is scarce information about the allergenic potential of amaranth proteins, either before or after food processing. Objective: To evaluate
实验方案
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
在测定酸性磷酸酶活性时,本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
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