P3895
磷酸酶(碱性) 来源于人类胎盘
≥10 units/mg solid (in glycine buffer)
别名:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-631-4
MDL number:
Specific activity:
≥10 units/mg solid (in glycine buffer)
Application
碱性磷酸酶可与抗体及其他蛋白质偶联而用于ELISA、Western blotting和组织化学检测。它通常用于使蛋白质和核酸去磷酸化。当需要高灵敏度时，可用于蛋白标记。碱性磷酸酶也可用于使DNA或RNA的5′-末端去磷酸化以防止自连。在用碱性磷酸酶去磷酸化后，DNA或RNA也可用放射性标记的磷酸盐（通过T4多核苷酸激酶）进行标记。
Biochem/physiol Actions
碱性磷酸酶可使蛋白质和核酸去磷酸化。人胎盘碱性磷酸酶（PLAP）具有独特的残基Glu-429。它是一种可被L-Phe、L-Trp和L-Leu无竞争性抑制的变构酶。PLAP是经过翻译后修饰的。磷脂酰肌醇特异性磷脂酶C可能参与到了PLAP的释放。PLAP可能在细胞分裂中发挥作用，并参与搭配母体IgG向胎儿的转移。
Other Notes
在pH 10.4，37 °C条件下，一个单位可在每分钟水解1 μ摩尔的4-硝基苯基磷酸酯。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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