form
saline suspension
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
~50 μg/mL binding capacity (DNA)
storage temp.
2-8°C
Application
Protamine-agarose is used in protein chromatography, affinity chromatography and specialty resins. Protamine-agarose has been used to determine that Pichia pastoris (methylotrophic yeast) provides a convenient heterologous system for the production of recombinant subunits of human type 2A protein phosphatase (PP2A). Protamine-agarose has also been used to purify and characterize a novel protamine kinase in HL60 cells as well as to study myocardial infarction.
Physical form
Suspension in 0.5 M NaCl containing 0.02% thimerosal
Disclaimer
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
V B Lokeshwar et al.
The Journal of biological chemistry, 264(32), 19318-19326 (1989-11-15)
Treatment of Swiss mouse 3T3 cells and human epidermoid carcinoma A431 cells with protamine at 37 degrees C increased the 125I-epidermal growth factor (EGF) binding activity at 4 degrees C. The effect of protamine on the increase of 125I-EGF binding
L Boscá et al.
The Biochemical journal, 290 ( Pt 3), 827-832 (1993-03-15)
The structural changes following the binding to protein kinase C (PKC) of activators that promote its translocation to lipid environments were studied by far-u.v. c.d. and intrinsic fluorescence measurements of the protein. In the absence of activators, PKC contained 40%
M Gschwendt et al.
FEBS letters, 307(2), 151-155 (1992-07-28)
Murine epidermis contains PKC zeta and eta as evidenced by the application of specific antisera. PKC zeta predominates in the cytosol and PKC eta in the particulate fraction. PKC zeta is shown to be present also in other murine tissues
Bruce M Brown et al.
Biochemistry, 41(46), 13526-13538 (2002-11-13)
In steps of protein purification of bovine retinal protein phosphatase 2A (PP2A), phosducin dephosphorylation activity peaks coelute with a PP2A enzyme complex, shown by peptide sequence analysis to contain a B' subunit, B56 epsilon. Other PP2A complexes with a slightly
J L Rosales et al.
Journal of cellular physiology, 164(3), 509-521 (1995-09-01)
The role of ras on protein kinase C (PKC) signaling was examined in two keratinocyte cell lines. Increasing the level of extracellular calcium from 0.15 mM to 1.0 mM induces some features of differentiation in the spontaneously immortalized HaCaT line
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