表单
saline suspension
基质
Cross-linked 4% beaded agarose
基质活化
cyanogen bromide
基质附着
amino
基质隔离区
1 atom
容量
~50 μg/mL binding capacity (DNA)
储存温度
2-8°C
应用
Protamine-agarose is used in protein chromatography, affinity chromatography and specialty resins. Protamine-agarose has been used to determine that Pichia pastoris (methylotrophic yeast) provides a convenient heterologous system for the production of recombinant subunits of human type 2A protein phosphatase (PP2A). Protamine-agarose has also been used to purify and characterize a novel protamine kinase in HL60 cells as well as to study myocardial infarction.
外形
Suspension in 0.5 M NaCl containing 0.02% thimerosal
免责声明
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
G M Walton et al.
Analytical biochemistry, 161(2), 425-437 (1987-03-01)
An efficient high yield three-step purification procedure for protein kinase C consisting of ion exchange, hydrophobic interaction, and substrate affinity chromatographies is described. Protein which appears homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis contains amino acid sequences, predicted from cDNA
Reversible block of the calcium release channel/ryanodine receptor by protamine, a heparin antidote.
P Koulen et al.
Molecular biology of the cell, 11(7), 2213-2219 (2000-07-11)
Channel activity of the calcium release channel from skeletal muscle, ryanodine receptor type 1, was measured in the presence and absence of protamine sulfate on the cytoplasmic side of the channel. Single-channel activity was measured after incorporating channels into planar
M Chedin et al.
Carcinogenesis, 18(8), 1463-1472 (1997-08-01)
Two different protein tyrosine kinases were detected in the cytosolic fraction of different human tumor tissues. After partial purification, the two enzymes, which were highly active in breast tumor tissues, were characterized. One of them, soluble tyrosine kinase-1 (STK-1), represents
M Gschwendt et al.
FEBS letters, 307(2), 151-155 (1992-07-28)
Murine epidermis contains PKC zeta and eta as evidenced by the application of specific antisera. PKC zeta predominates in the cytosol and PKC eta in the particulate fraction. PKC zeta is shown to be present also in other murine tissues
V B Lokeshwar et al.
The Journal of biological chemistry, 264(32), 19318-19326 (1989-11-15)
Treatment of Swiss mouse 3T3 cells and human epidermoid carcinoma A431 cells with protamine at 37 degrees C increased the 125I-epidermal growth factor (EGF) binding activity at 4 degrees C. The effect of protamine on the increase of 125I-EGF binding
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