生物来源
rabbit muscle
表单
lyophilized powder
比活
0.2-1 inhibitor U/μg protein
组成
Protein, ~95% biuret
储存温度
2-8°C
生化/生理作用
Binds to the catalytic subunit of cyclic-AMP dependent protein kinase, displacing the regulatory subunit and inhibiting phosphorylating activity. The cAMP binding capacity of the regulatory subunit is enhanced. The amino acid sequence deduced from cDNA is 100% homologous with human PKI.
包装
Package size based on protein content.
外形
Lyophilized powder containing 5% potassium phosphate buffer salts
制备说明
Fractionated essentially by procedure of Walsh, D.A., et al., J. Biol. Chem., 246, 1977 (1971), through TCA precipitation.
分析说明
粗品
其他说明
One unit will transfer 1.0 picomole (10-12 mol) of phosphate from γ-32P-ATP to hydrolyzed and partially dephosphorylated casein (Product No. C 4765) per min at pH 6.5 at 30 °C.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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J Stern et al.
The Journal of general physiology, 85(2), 157-169 (1985-02-01)
The effect of intracellular nucleotides on the rate of spontaneous quantum bumps in Limulus ventral photoreceptors has been examined. Internal dialysis of photoreceptors with solutions lacking nucleotide leads to an elevation of the quantum bump rate that can be reversed
Protein phosphorylation and hydrogen ions modulate calcium-induced closure of gap junction channels.
R O Arellano et al.
Biophysical journal, 57(2), 363-367 (1990-02-01)
The regulation of the cell-to-cell pathway formed by gap junctions seems to involve the interaction of the junctional channels with either calcium or hydrogen ions, as well as protein phosphorylation and calmodulin. These mechanisms of junctional regulation have been considered
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