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Merck
CN

P5656

Modified Lowry Kit for Protein Determination (Peterson′s Modification of Micro-Lowry Method)

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UNSPSC Code:
12352200

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usage

sufficient for ~200 assays

IVD

for in vitro diagnostic use

Application

Uses sodium dodecyl sulfate for dissolving lipoproteins; offers excellent recovery of membrane proteins.

Biochem/physiol Actions

An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve

Features and Benefits

•Complete kit for protein assays
•Most widely cited protein assay
•Less protein to protein variation than other assays
•Contains reagents that can remove interfering substances

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说明
  • DOC solution 20 mL
  • Folin-Ciocalteu phenol reagent 1 bottle
  • Lowry Reagent 5 bottle
  • Protein standard 5 vial(s)
  • TCA solution 20 mL

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Assay of proteins in the presence of interfering materials.
A Bensadoun et al.
Analytical biochemistry, 70(1), 241-250 (1976-01-01)
A simplification of the protein assay method of Lowry et al. which is more generally applicable.
G L Peterson
Analytical biochemistry, 83(2), 346-356 (1977-12-01)
Protein measurement with the Folin phenol reagent.
O H LOWRY et al.
The Journal of biological chemistry, 193(1), 265-275 (1951-11-01)

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