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Merck
CN

P6022

Protein Kinase A Regulatory subunit from bovine heart

secondary activity: 50-100 (units per μg protein), lyophilized powder

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UNSPSC Code:
12352200
MDL number:
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form

lyophilized powder

mol wt

dimer 400 residues (each chain)

capacity

5-10 nmol/mg, protein binding capacity (cyclic-AMP)(of cyclic AMP bound at pH 7.0, 0 °C at 64 nM [cyclic-AMP].)

storage temp.

−20°C

Packaging

Package size based on inhibitory units.

Physical form

Lyophilized powder containing ≤ 1% protein with sucrose and phosphate buffer salts as stabilizer

Preparation Note

For full activity, reconstitute in water with 6 mg/ml DTT.

Analysis Note

Residual phosphorylating activity of approx. 0.08 unit per μg protein is found at pH 6.5 at 30 °C.

Other Notes

One unit will inhibit one unit of "catalytic subunit" phosphorylating activity in the absence of cyclic-AMP.


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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R O Arellano et al.
Biophysical journal, 57(2), 363-367 (1990-02-01)
The regulation of the cell-to-cell pathway formed by gap junctions seems to involve the interaction of the junctional channels with either calcium or hydrogen ions, as well as protein phosphorylation and calmodulin. These mechanisms of junctional regulation have been considered