苦味酸 溶液

Picric acid is a polynitrated aromatic acid. It dissolves in water to form a solution with low pH (pH 2.0). It interacts with basic groups of proteins, forms salts and coagulates them. Fixation with picric acid gives a bright stain, but there might be a loss of nucleic acids due to the low pH of the solution.
Picric acid is anionic and has a low molecular weight.

Picric acid is commonly used as a fixative and staining reagent in forensic and histological applications. It is a general cytoplasmic stain, connective tissue fiber stain, and a fixative for tissues.

• It is a component of picrosirius red which is used to stain collagen.
• Bouin’s fluid which also comprises picric acid is a fixative for nuclei and connective tissue.
• It can be used as a colorimetric reagent in creatinine determination assay.