P6772
碱性磷酸酶 来源于牛肠粘膜
lyophilized powder, ≥2,000 DEA units/mg protein
别名:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum), 碱性磷酸酶
form
lyophilized powder
specific activity
≥2,000 DEA units/mg protein
mol wt
dimer ~160 kDa
composition
Protein, 25-45% biuret
storage temp.
−20°C
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General description
牛肠碱性磷酸酶为二聚膜衍生糖蛋白。至少存在三种同工酶，每个单体通常拥有两个 N-连接多糖和一个或多个 O-连接多糖。2 该酶需要锌以及镁或钙二价离子才能显示活性。
牛肠道碱性磷酸酶是一种二聚化的膜衍生糖蛋白。其存在至少三种异构体，每个单体通常具有两个N-连接的和一个或多个O-连接的聚糖。
Application
推荐用于抗体和蛋白质缀合的高比活性等级。
碱性磷酸酶可用于酪蛋白和其他蛋白质的去磷酸化。碱性磷酸酶还可用于 DNA 或 RNA 的 5′-末端的去磷酸化，以防止自我连接。经碱性磷酸酶的去磷酸化作用后，DNA 或 RNA 还可被放射性标记磷酸盐标记（通过 T4 多核苷酸激酶）。
Biochem/physiol Actions
该酶对醇类、胺类、焦磷酸盐以及酚类的磷酸酯具有广泛的特异性。常用于蛋白质和核酸的去磷酸化。
Physical form
Lyophilized powder containing Tris-Citrate buffer salts
Preparation Note
Affinity purified. Prepared from P5521, Type VII-S.
Analysis Note
包装规格取决于 DEA 单位
Other Notes
在pH 9.8,37℃条件下，一个DEA单位可每分钟水解1 μmole的4-硝基苯基磷酸酯。（一个甘氨酸单位相当于~3个DEA单位）
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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