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Merck
CN

P7973

SP6 Promoter Primer Set

别名:

5′-ATT TAG GTG ACA CTA TAG-3′

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UNSPSC Code:
41106305
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storage temp.

2-8°C

Application

Functionally tested for use in fluorescence-detection automated sequencing.
SP6 Promoter Primer Set is a single-stranded oligonucleotide with 5′-hydroxyl and 3′-hydroxyl ends and a selection of four fluorescent lables for use in polymerase chain reaction protocols (PCR).

Packaging

Packaged in amber tubes to protect from light.

Analysis Note

Tested for purity with HPLC, PAGE, and OD.

Other Notes

Set of four vials of primer, each individually labeled at the 5′-end with:
FAM: (5[6]-carboxyfluorescein)
JOE: (6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein)
ROX: (5[6]-carboxy-X-rhodamine), and
TAMRA: (5[6]-carboxytetramethylrhodamine.
Supplied as solutions, 50 μl each, in tris-EDTA buffer, pH 7.5, at 1 picomole/μl

存储类别

10 - Combustible liquids

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High-throughput sequencing of PCR products tagged with universal primers using 454 life sciences systems.
Daigle D, Simen BB, Pochart P.
Current Protocols in Molecular Biology, Unit 7-Unit 7 (2011)
Angela Capece et al.
International journal of food microbiology, 144(1), 187-192 (2010-10-12)
The present research studied Saccharomyces cerevisiae yeasts isolated from Nero d'Avola grapes, collected in different areas of the Sicily region. RAPD-PCR analysis with M13 primer was used for preliminary discrimination among 341 S. cerevisiae isolates. Inoculated fermentations with S. cerevisiae
Jacquelina Williams-Woods et al.
Journal of virological methods, 178(1-2), 253-257 (2011-10-04)
Human norovirus (HuNoV) and hepatitis A (HAV) are recognized as leading causes of non-bacterial foodborne associated illnesses in the United States. DNA sequencing is generally considered the standard for accurate viral genotyping in support of epidemiological investigations. Due to the

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