Anti-Protein Phosphatase 1α antibody produced in rabbit

Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. PP1 catalyzes a wide range of protein dephosphorylation reactions in a tightly regulated manner and is expressed abundantly in the brain. PP1 has broad functions covering glycogen metabolism, protein synthesis, cell cycle and growth and muscle contractility. PP1 forms exclusive complexes with >50 regulatory subunits that allows for restricted subcellular location and thereby distinct cellular functions. There are 3 isoforms of PP1 (α, β and γ1) with 90% homology. PP1α is expressed in brain and muscle.
Anti-Serine/threonine protein phosphatase 1α specifically recognizes PP1α isoform and recognizes an epitope within its catalytic subunit (36 kDa) in human, mouse, rat and bovine.

synthetic peptide corresponding to amino acids 316-328 of the human protein phosphatase 1α (catalytic subunit) on a synthetic MAP carrier.

A minimum dilution of 1:2000 of Anti-PP1α is used for detection by immunoblotting in whole cell extracts of A431, human epidermoid carcinoma cell line. The antibody is also suitable for protein microarray.

By immunoblotting, the antibody specifically reacts with protein phosphatase 1α and recognizes an epitope within its catalytic subunit. The sequence is identical in rabbit PP1α.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.