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Merck
CN

P8547

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–R-Phycoerythrin antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

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UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
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biological source

sheep

conjugate

phycoerythrin (R-PE) conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect immunofluorescence: 1:20

shipped in

wet ice

storage temp.

2-8°C

General description

Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Application

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-R-Phycoerythrin antibody produced in sheep was used in FISH analysis of human lymphocytes.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. Conjugation of R-Phycoerythrin to the antibody enables the use in immunofluorescent techniques.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.1 mM EDTA, 1 mM iodoacetamide, 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human samples.

Other Notes

Antibody adsorbed with human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

新产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Meilin Tang et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 32(1), 86-99 (2013-07-11)
Low efficiency of cardiomyocyte (CM) differentiation from embryonic stem (ES) cells limits their therapeutic use. The objective of this study was to investigate the effect of baicalin, a natural flavonoid compound, on the in vitro cardiac differentiation of murine ES
Aleksandra Nowak et al.
International journal of oncology, 51(2), 668-676 (2017-06-29)
The process of angiogenesis based on new vessel formation within the tumour area plays a significant role in the progression of breast cancer. Nestin is an intermediate filament protein and participates in the cytoskeleton organization. Nestin expression in the endothelium
Gregory T Kennedy et al.
Immunology letters, 166(1), 28-35 (2015-05-23)
Recent studies suggest that immunotherapy may offer a promising treatment strategy for early-stage malignant pleural mesothelioma (MPM), but advanced tumor burden may limit the efficacy of immunotherapy. Therefore, we hypothesized that surgical cytoreduction could restore the efficacy of vaccine-based immunotherapy
Ying Cheng et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 32(4), 789-800 (2013-10-02)
It is important to screen and identify chemical compounds to improve the efficiency of cardiac differentiation and specialization of embryonic stem (ES) cells. The objective of this study was to investigate the effect of puerarin, a natural phytoestrogen, on the
Julia Tchou et al.
Breast cancer research and treatment, 133(2), 799-804 (2012-03-16)
Mesothelin is a cell-surface glycoprotein present on mesothelial cells and elicits T cell responses in a variety of cancers including pancreatic, biliary and ovarian cancer. Breast cancer is not known to express mesothelin. We postulated that mesothelin may be a

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