biological source
bovine brain
form
lyophilized powder
specific activity
1-5 units/mg protein (without added calmodulin)
mol wt
~60 kDa
composition
Protein, 4.0-6.0% Lowry
UniProt accession no.
foreign activity
2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase ≤0.1%, 5′-ATPase ≤0.2%, inorganic pyrophosphatase ≤0.8%
storage temp.
−20°C
Gene Information
cow ... PDE1A(281969)
General description
Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca2+ second-messenger systems.
Application
Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.
Biochem/physiol Actions
水解环单磷酸核苷酸（如cAMP 和 cGMP）的 3′,5′-磷酸二酯，生成相应的 5′-单磷酸核苷酸。
Packaging
根据活化单位确定包装尺寸
Physical form
含 Tris-HCl 缓冲盐和乳糖的冻干粉
Preparation Note
已耗尽钙调蛋白的亲和纯化制剂。
用 50% 甘油复原。在 -20°C 下储存，酶的总活化单位可保持至少 5 天的恒定状态。但是，钙调蛋白不足时的活性可增加至 200%。如果在 4°C 下储存，激活时的活性和钙调蛋白不足时的活性都将在 24 小时内下降约 30%。
Analysis Note
由 Warburg-Christian 法测定的蛋白质
Other Notes
在 pH 7.5 和 30°C 下，一单位本品每分钟可将 1.0μmol 3′,5′-cAMP 水解为 5′-AMP。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Drummond, G.I. And Yamamoto, M. et al.
The Enzymes (1971)
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Kevin J. Ziolo
Infection (Munich) (2014)
M D Houslay
Seminars in cell & developmental biology, 9(2), 161-167 (1998-05-26)
Cyclic AMP has provided the paradigm for the second messenger concept. Recent evidence has identified a complex array of isoforms of the enzymes involved in generation, destroying and transmitting the effects of this second messenger. These proteins provide a sophisticated
P D Calvert et al.
The Journal of general physiology, 111(1), 39-51 (1998-02-14)
Light adaptation in vertebrate photoreceptors is thought to be mediated through a number of biochemical feedback reactions that reduce the sensitivity of the photoreceptor and accelerate the kinetics of the photoresponse. Ca2+ plays a major role in this process by
Reciprocal regulation of calcium dependent and calcium independent cyclic AMP hydrolysis by protein phosphorylation
Kok-Long Ang Ferenc A. Antoni
Journal of Neurochemistry (2002)
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