concentration
20 ×
compatibility
for use with Sigma-Aldrich TruPAGE Precast Gels
storage temp.
room temp
Application
Tris-MOPS™SDS Express 电泳缓冲液用于通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离蛋白质。
Features and Benefits
TruPAGE Tris-MOPS SDS Express 运行缓冲液专门配制用于 TruPAGE 预制胶。该运行缓冲液将为较小的MW蛋白提供更高的分离度，并明显缩短运行时间。该运行缓冲液需要添加1X工作浓度的TruPAGE抗氧化剂 (PCG3007)，以防止电泳时还原的蛋白质样品中的蛋白质重新氧化。 请参考凝胶迁移图以选择适合您实验需要的预制凝胶和运行缓冲液组合。
Legal Information
TruPAGE is a trademark of Sigma-Aldrich Co. LLC
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Detection of phosphorylated Akt and MAPK in cell culture assays.
Molgaard S, et al.
MethodsX, 3, 386-398 (2016)
Simon Molgaard et al.
MethodsX, 3, 386-398 (2016-06-09)
This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite
John R Christin et al.
Cell reports, 31(10), 107742-107742 (2020-06-11)
Lineage plasticity is important for the development of basal-like breast cancer (BLBC), an aggressive cancer subtype. While BLBC is likely to originate from luminal progenitor cells, it acquires substantial basal cell features and contains a heterogenous collection of cells exhibiting
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