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Merck
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PROT20

ProteoPrep® 20血浆免疫去除试剂盒

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UNSPSC Code:
12352200

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shipped in

wet ice

storage temp.

2-8°C

Application

ProteoPrep 20血浆免疫去除试剂盒可从为进一步的蛋白质组分析而制备的人血浆或血清样本中特异性去除20种最高丰度的蛋白质。无论您采用二维还是三维电泳，一维还是多维色谱，亦或是直接进行质谱，这种去除方法均可实现更深的血浆蛋白质组渗透率。这一方法基于小分子量重组免疫亲和配体与可在支撑物上进行高密度偶联的传统抗体的独特偶联过程。可选的配体确保了最佳的特异性。对于人血浆或血清，其典型的总蛋白去除率为97-98%（质量）。

Features and Benefits

• 提高可视化低丰度蛋白和生物标志物的能力 – 可有效去除>99% 的二十种最高丰度蛋白质，可将特定蛋白载量提高50倍，从而增加了低丰度蛋白可视化的几率

• 独特的抗体亲和介质 – 新型高密度抗体亲和介质显示出了更高的特异性，可以减少污染，去除污染物能力相对于所有现有产品大幅提升

• 方便的离心柱形式 – 快速去除污染物和回收，提高了通量和验证能力，成为了所有蛋白质组实验室的首选

General description

Proteoprep 20是一种免疫去除试剂盒，可从血浆或血清中去除20种高丰度干扰蛋白，从而分析低丰度蛋白。Proteoprep 20 可去除的蛋白：白蛋白、IgG、转铁蛋白、纤维蛋白原、gA, α2巨球蛋白、IgM, α1抗胰蛋白脢、补体蛋白C3、 触珠蛋白、载脂蛋白A1、载脂蛋白A3和载脂蛋白B；α1酸性糖蛋白、铜蓝蛋白、补体蛋白C4、补体蛋白C1q、IgD、前白蛋白和纤维蛋白溶酶原。

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

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GHS07

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Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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  1. Can the Product PROT20, ProteoPrep Plasma Immunodepletion Kit, columns be re-used?

    With proper cleaning and storage, each column may be reused at least 100 times.

  2. The Product PROT20, ProteoPrep Plasma Immunodepletion Kit, bulletin indicates that 8 microliters of plasma are loaded on to the column. I have more than 8 microliters of plasma. Can I load greater than 8 microliters according to the procedure? 

    We optimized the protocol for dilution of about 8 microliters of plasma to 100 microliters with the 1× Equilibration Buffer. It is probably best to dilute 8 or so microliters at a time and load on the column for depletion. This process can be repeated for the full sample.

  3. Can I use Product PROT20, ProteoPrep Plasma Immunodepletion Kit, columns on plasma from other species, e.g. monkey?

    The antibodies on the PROT20 column are specific for depletion of human plasma proteins. We would not recommend this product for other species. The PROTBA is a more economical possibility to try, even though that depletes only two proteins from serum.

  4. Are the Product PROT20, ProteoPrep Plasma Immunodepletion Kit, columns available as stand-alone products?

    The columns are not available separately from the kit. The only kit components available separately are CLS8160 and M0286. We offer a smaller scale kit, PROT20S, which has one column instead of 3.

  5. Is there a point in the protocol for Product PROT20, ProteoPrep Plasma Immunodepletion Kit, where I can stop in the middle?

    In general, it is best to complete the depletion procedure once it is started. However, the customer can, if necessary, stop the procedure after the primary depletion and store the depleted samples frozen. The customer can then elute the bound proteins from the resin later and reuse it for re-depletion of the sample.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Tara K Sigdel et al.
Frontiers in medicine, 4, 80-80 (2017-07-04)
Identification and use of non-invasive biomarkers for kidney transplantation monitoring is an unmet need. A total of 121 biobanked sera collected from 111 unique kidney transplant (KT) patients (children and adolescent) and 10 age-matched healthy normal controls were used to
Removal of albumin from multiple human serum samples.
K Rengarajan et al.
BioTechniques, 20(1), 30-32 (1996-01-01)
Stephanie D Byrum et al.
PloS one, 12(3), e0174771-e0174771 (2017-03-30)
Acute radiation syndrome (ARS) is a complex multi-organ disease resulting from total body exposure to high doses of radiation. Individuals can be exposed to total body irradiation (TBI) in a number of ways, including terrorist radiological weapons or nuclear accidents.
Jon M Jacobs et al.
Journal of proteome research, 4(4), 1073-1085 (2005-08-09)
Candidate proteomic biomarker discovery from human plasma holds both incredible clinical potential as well as significant challenges. The dynamic range of proteins within plasma is known to exceed 10(10), and many potential biomarkers are likely present at lower protein abundances.
Stéphane Roche et al.
Journal of proteomics, 72(6), 945-951 (2009-04-04)
Depletion of major blood proteins is one of the most promising approaches to access low abundant biomarkers using proteomics. Immunocapture columns often used for this purpose exist in different formats depending on the number of major proteins removed. In this
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Absolute Quantifi cation of the Lower Abundance Proteome Through Immunoaffi nity Depletion of the Twenty Most Abundant Proteins in Human Serum
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We have developed a novel, high-binding capacity, antibodybased resin for the depletion of twenty high abundance proteins in human plasma. The technology is a significant improvement over existing kits that remove 2, 6, or 12 proteins.

User Protocol
ProteoPrep 20 Plasma Immunodepletion Kit
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