Application
Restrictocin from Aspergillus restrictus is used to inhibit protein synthesis. It may be useful as a component of immunotoxins . Product R0389 is provided as a lyophilized powder.
Biochem/physiol Actions
Restrictocin from Aspergillus restrictus is a ribosome-inactivating protein that acts by specifically cleaving rRNA. Approximately 70% of the protein is active restrictocin. Restrictocin shares a high degree of amino acid sequence homology with mitogillin, a ribotoxin that cleaves a single phosphodiester bond of the 29S rRNA of eukaryotic ribosomes . Restrictocin is thought to be activated during the process of secretion .
Physical form
Lyophilized from 0.3 mL of 0.5% NaCl
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
高风险级别生物产品--毒素类产品
此项目有
Alexei V Korennykh et al.
Biochemistry, 46(44), 12744-12756 (2007-10-13)
Restrictocin is a site-specific endoribonuclease that inactivates ribosomes by cleaving the sarcin/ricin loop (SRL) of 23S-28S rRNA. Here we present a kinetic and thermodynamic analysis of the SRL cleavage reaction based on monitoring the cleavage of RNA oligonucleotides (2-27-mers). Restrictocin
M Weig et al.
Journal of clinical microbiology, 39(5), 1721-1730 (2001-04-28)
During human infection, Aspergillus fumigatus secretes a 18-kDa protein that can be detected as an immunodominant antigen in the urine of infected patients. Recently, this protein was shown to be mitogillin, a ribotoxin that cleaves a single phosphodiester bond of
R Kappe et al.
Mycoses, 47 Suppl 1, 55-59 (2005-01-26)
The clinical significance of Aspergillus antibody assays for the diagnosis of invasive aspergillosis (IA) is unclear. In two studies, three different antibody assays were evaluated with patients suffering from proven IA: (i) a commercial haemagglutination test (HAT), (ii) a commercial
S K Nayak et al.
Biochemistry, 40(31), 9115-9124 (2001-08-02)
Restrictocin, a member of the fungal ribotoxin family, specifically cleaves a single phosphodiester bond in the 28S rRNA and potently inhibits eukaryotic protein synthesis. Residues Tyr47, His49, Glu95, Phe96, Pro97, Arg120, and His136 have been predicted to form the active
X Yang et al.
Nature structural biology, 8(11), 968-973 (2001-10-31)
The cytotoxin sarcin disrupts elongation factor binding and protein synthesis by specifically cleaving one phosphodiester bond in ribosomes. To elucidate the molecular basis of toxin action, we determined three cocrystal structures of the sarcin homolog restrictocin bound to different analogs
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持