生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
克隆
polyclonal
表单
buffered aqueous glycerol solution
用途
sufficient for 10 blots
种属反应性
rat, human, mouse
技术
western blot: 1:1,000 using HEK293 cells overexpressing c-Raf
运输
wet ice
储存温度
−20°C
基因信息
human ... RAF1(5894)
mouse ... Zhx2(387609)
一般描述
Raf-1 is a serine/threonine kinase that is activated by tyrosine kinases . Raf-1 inhibits Ras signaling and subsequently modulates cell proliferation and development . c-Raf expression has been linked to the pathogenesis of lung carcinoma . Anti-phospho-c-Raf pTyr340/341 antibody is specific for human c-Raf that is phosphorylated on tyrosines 340 and 341. Although the product has not been tested for cross-reactivity, the antibody is expected to recognize mouse and rat c-Raf (100% homologous).
免疫原
synthetic phosphopeptide derived from a region of human Raf that contains tyrosines 340 and 341.
应用
Anti-phospho-c-Raf pTyr340/341 antibody is suitable for use in western blot (1:1,000 using HEK293 cells overexpressing c-Raf).
外形
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free), 50% glycerol, and 0.05% sodium azide.
法规信息
新产品
此项目有
J A Thorson et al.
Molecular and cellular biology, 18(9), 5229-5238 (1998-08-26)
By binding to serine-phosphorylated proteins, 14-3-3 proteins function as effectors of serine phosphorylation. The exact mechanism of their action is, however, still largely unknown. Here we demonstrate a requirement for 14-3-3 for Raf-1 kinase activity and phosphorylation. Expression of dominant
D K Morrison
Molecular reproduction and development, 42(4), 507-514 (1995-12-01)
Raf-1 is a key protein involved in the transmission of developmental and proliferative signals generated by receptor and nonreceptor tyrosine kinases. Biochemical and genetic studies have demonstrated that Raf-1 functions downstream of activated tyrosine kinases and Ras and upstream of
J R Fabian et al.
Molecular and cellular biology, 13(11), 7170-7179 (1993-11-01)
The serine/threonine kinase activity of the Raf-1 proto-oncogene product is stimulated by the activation of many tyrosine kinases, including growth factor receptors and pp60v-src. Recent studies of growth factor signal transduction pathways demonstrate that Raf-1 functions downstream of activated tyrosine
Rafael B Blasco et al.
Cancer cell, 19(5), 652-663 (2011-04-26)
We have investigated the role of individual members of the Raf/Mek/Erk cascade in the onset of K-Ras oncogene-driven non-small cell lung carcinoma (NSCLC). Ablation of Erk1 or Erk2 in K-Ras oncogene-expressing lung cells had no significant effect due to compensatory
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