R3251
D-核酮糖-5-磷酸-3-表异构酶 来源于面包酵母（酿酒酵母）
lyophilized powder, 50-100 units/mg protein (modified Warburg-Christian)
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关于此项目
Specific activity:
50-100 units/mg protein (modified Warburg-Christian)
Biological source:
bakers yeast
biological source
bakers yeast
form
lyophilized powder
specific activity
50-100 units/mg protein (modified Warburg-Christian)
foreign activity
phosphoriboisomerase, alcohol dehydrogenase, transketolase, and transaldolase <0.1%
storage temp.
−20°C
Application
D-Ribulose-5-phosphate 3-Epimerase is an enzyme that converts the reversible conversion of D-ribulose 5-phosphate into D-xylulose 5-phosphate, which is important for the cellular response against oxidative stress . D-Ribulose-5-phosphate 3-Epimerase is involved in the pentose phosphate pathway, pentose and glucuronate interconversions and carbon fixation. Product R3251 is from baker′s yeast and is provided as a lyophilized powder. It is useful in enzyme systems requiring low sulfate.
Biochem/physiol Actions
RPE is a metalloenzyme and has been shown to use the divalent Zn2+ ion predominantly for catalysis. Human D-ribulose-5-phosphate 3-epimerase (hRPE) has been shown to use Fe2+ for catalysis .
Physical form
基本上无硫酸盐的冻干粉：约含35% 的柠檬酸缓冲盐
Other Notes
在 pH 7.7 和 25°C 下，与转酮醇酶、α-甘油磷酸脱氢酶和磷酸丙糖异构酶偶联时，一单位每分钟可将 1μmol 的 D-核酮糖-5-磷酸转化为 D-木酮糖-5-磷酸。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Stefan Jelakovic et al.
Journal of molecular biology, 326(1), 127-135 (2003-01-28)
Cytosolic D-ribulose-5-phosphate 3-epimerase from rice was crystallized after EDTA treatment and structurally elucidated by X-ray diffraction to 1.9A resolution. A prominent Zn(2+) site at the active center was established in a soaking experiment. The structure was compared with that of
Jason M Sobota et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(13), 5402-5407 (2011-03-16)
H(2)O(2) is commonly generated in biological habitats by environmental chemistry and by cellular immune responses. H(2)O(2) penetrates cells, disrupts metabolism, and blocks growth; it therefore is of interest to identify the major cellular molecules that H(2)O(2) damages and the strategies
S Graupner et al.
Biomolecular engineering, 17(1), 11-16 (2000-10-24)
A series of expression vectors for gram-negative bacteria was constructed which combine broad-host-range, inducible expression from the tac promoter and diverse antibiotic resistance determinants. The tac promoter activity and the repression by lacIq can be quantitated with a separate test