R3251
D-核酮糖-5-磷酸-3-表异构酶 来源于面包酵母（酿酒酵母）
lyophilized powder, 50-100 units/mg protein (modified Warburg-Christian)
生物来源
bakers yeast
表单
lyophilized powder
比活
50-100 units/mg protein (modified Warburg-Christian)
异质活性
phosphoriboisomerase, alcohol dehydrogenase, transketolase, and transaldolase <0.1%
储存温度
−20°C
应用
D-Ribulose-5-phosphate 3-Epimerase is an enzyme that converts the reversible conversion of D-ribulose 5-phosphate into D-xylulose 5-phosphate, which is important for the cellular response against oxidative stress . D-Ribulose-5-phosphate 3-Epimerase is involved in the pentose phosphate pathway, pentose and glucuronate interconversions and carbon fixation. Product R3251 is from baker′s yeast and is provided as a lyophilized powder. It is useful in enzyme systems requiring low sulfate.
生化/生理作用
RPE is a metalloenzyme and has been shown to use the divalent Zn2+ ion predominantly for catalysis. Human D-ribulose-5-phosphate 3-epimerase (hRPE) has been shown to use Fe2+ for catalysis .
外形
基本上无硫酸盐的冻干粉：约含35% 的柠檬酸缓冲盐
其他说明
在 pH 7.7 和 25°C 下，与转酮醇酶、α-甘油磷酸脱氢酶和磷酸丙糖异构酶偶联时，一单位每分钟可将 1μmol 的 D-核酮糖-5-磷酸转化为 D-木酮糖-5-磷酸。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Jason M Sobota et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(13), 5402-5407 (2011-03-16)
H(2)O(2) is commonly generated in biological habitats by environmental chemistry and by cellular immune responses. H(2)O(2) penetrates cells, disrupts metabolism, and blocks growth; it therefore is of interest to identify the major cellular molecules that H(2)O(2) damages and the strategies
Stefan Jelakovic et al.
Journal of molecular biology, 326(1), 127-135 (2003-01-28)
Cytosolic D-ribulose-5-phosphate 3-epimerase from rice was crystallized after EDTA treatment and structurally elucidated by X-ray diffraction to 1.9A resolution. A prominent Zn(2+) site at the active center was established in a soaking experiment. The structure was compared with that of
S Graupner et al.
Biomolecular engineering, 17(1), 11-16 (2000-10-24)
A series of expression vectors for gram-negative bacteria was constructed which combine broad-host-range, inducible expression from the tac promoter and diverse antibiotic resistance determinants. The tac promoter activity and the repression by lacIq can be quantitated with a separate test
Dante A Maugeri et al.
FEMS microbiology letters, 234(1), 117-123 (2004-04-28)
The pentose phosphate pathway has been studied in Trypanosoma cruzi, Clone CL Brener. Functioning of the pathway was demonstrated in epimastigotes by measuring the evolution of (14)CO(2) from [1-(14)C] or [6-(14)C]D-glucose. Glucose consumption through the PPP increased from 9.9% to
Lan Hu et al.
Biochemical and biophysical research communications, 320(3), 648-655 (2004-07-09)
Replication of the human genome requires the activation of thousands of replicons distributed along each one of the chromosomes. Each replicon contains an initiation, or origin, site, at which DNA synthesis begins. However, very little information is known about the
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