grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition sequence: 5′-G/GTNACC-3′
Cutting results: a 2-10-fold BstE II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: This enzyme cannot be inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold BstE II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: This enzyme cannot be inactivated at 65 °C for 15 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 7.4, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA and 50% [v/v] glycerol
Other Notes
Comment: BstEII results in 10-15% activity at 37°C.
One unit is the enzyme activity that completely cleaves 1 mg of λDNA in 1 hour at 60 °C in the 1x reaction buffer in a total volume of 50 mL.
Supplied with 10x Reaction Buffer BstE II
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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C Kessler et al.
Gene, 47(1), 1-153 (1986-01-01)
The properties and sources of all known restriction endonucleases and methylases are listed. The enzymes are cross-indexed (Table I), classified according to their recognition sequence homologies (Table II), and characterized within Table II by the cleavage and methylation positions, the
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
TALENs and ZFNs are associated with different mutation signatures.
Yongsub Kim et al.
Nature methods, 10(3), 185-185 (2013-02-12)
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
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