等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
生化/生理作用
Recognition sequence: 5′-G/GTNACC-3′
Cutting results: a 2-10-fold BstE II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: This enzyme cannot be inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold BstE II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: This enzyme cannot be inactivated at 65 °C for 15 minutes.
外形
Solution in 10 mM Tris-HCl, pH 7.4, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA and 50% [v/v] glycerol
其他说明
Comment: BstEII results in 10-15% activity at 37°C.
One unit is the enzyme activity that completely cleaves 1 mg of λDNA in 1 hour at 60 °C in the 1x reaction buffer in a total volume of 50 mL.
Supplied with 10x Reaction Buffer BstE II
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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