等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
应用
DdeI is a DNA restriction enzyme used in molecular biology research that cleaves the recognition sequence 5′-C/TNAG-3′, generating 5′-cohesive termini.
生化/生理作用
Recognition sequence: 5′-C/TNAG-3′
Ligation and recutting results: After 2-10-fold Dde I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Completed after 65 °C incubation for 20 minutes.
Comment: Single-stranded DNA cleaves slowly.
Ligation and recutting results: After 2-10-fold Dde I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Completed after 65 °C incubation for 20 minutes.
Comment: Single-stranded DNA cleaves slowly.
外形
Solution in 10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 50 mM NaCl, 1 mM DTT, 0.01% Triton X- 100 (v/v), 500μg BSA, 50% glycerol (v/v) at 4°C
其他说明
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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