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Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
RsaI is a restriction endonuclease that is used in molecular biology methods to cut DNA at the recognition sequence 5′-GT/AC-3′ to generate DNA fragments with blunt termini.
Biochem/physiol Actions
Recognition sequence: 5′-GT/AC-3′
Cutting results: a 2-10-fold Rsa I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Cutting results: a 2-10-fold Rsa I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Physical form
Solution in 40 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.25% polydocanol (v/v) at 4 °C
Other Notes
Comment: Rsa I cuts single-stranded DNA poorly.
Supplied with 10x Restriction Enzyme Buffer SL (B3782).
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Delphine Boucher et al.
FEMS microbiology ecology, 70(1), 66-78 (2009-07-23)
This study examined the effects of temporal changes in bacterial community composition (BCC) and environmental factors on potential ectoenzymatic activities (alpha-glucosidase, beta-glucosidase, alkaline phosphatase and leucine aminopeptidase) in a lacustrine ecosystem (Sep reservoir, France). BCC was assessed by terminal restriction
Cécile Lepère et al.
Applied and environmental microbiology, 72(4), 2971-2981 (2006-04-07)
The structure and dynamics of small eukaryotes (cells with a diameter less than 5 microm) were studied over two consecutive years in an oligomesotrophic lake (Lake Pavin in France). Water samples were collected at 5 and 30 m below the
S P Lynn et al.
Journal of bacteriology, 142(2), 380-383 (1980-05-01)
A new type II sequence-specific endonuclease, RsaI, has been identified from Rhodopseudomonas sphaeroides strain 28/5. An RsaI purification scheme that yields enzyme which is free of contaminating exonuclease and phosphatase activities after a single column fractionation has been developed. The