R5029
Anti-RAD1, C-terminal antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
别名:
Anti-Cell Cycle Checkpoint Protein hRad1, Anti-REC1-DNA Repair Exonuclease, Anti-Rad1-like DNA Damage Checkpoint Protein
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IP, WB
Citations:
3
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 30 kDa
species reactivity
human
technique(s)
immunoprecipitation (IP): 2-4 μL using 293-T cell lysates, microarray: suitable, western blot: 1:500-1:1,000 using lysates of HL-60 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAD1(5810)
General description
RAD1 checkpoint DNA exonuclease/RAD1 is located on human chromosome 5p13.2. It belongs the Rad family proteins.
Immunogen
synthetic peptide corresponding to amino acids 267-282 of human RAD1, conjugated to KLH via an N-terminal added lysine residue. The immunizing peptide is present in RAD1A and RAD1B (isoforms 1 and 2).
Application
Anti-RAD1, C-terminal antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Anti RAD1 (C-terminal) specifically recognizes RAD1.
RAD1 checkpoint DNA exonuclease/RAD1 can form a stable radioresponsive checkpoint complex commonly known as 9-1-1, which participates in cellular responses to DNA damage or inhibition of DNA synthesis. Rad1 can act as a DNA damage sensor. RAD1 exhibits terminal exonuclease activity on double-stranded DNA with a preference for 3′ ends. It can regulate specifically the expansion of minisatellite tracts.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
RAD1 Controls the Meiotic Expansion of the Human HRAS1 Minisatellite in Saccharomyces cerevisiae
Jauert PA, et al.
Molecular and Cellular Biology, 22(3), 953-964 (2002)
Human hRad1 but not hRad9 protects hHus1 from ubiquitin-proteasomal degradation
Hirai I, et al.
Oncogene, 23(30), 5124-5130 (2004)
U K Marathi et al.
Genomics, 54(2), 344-347 (1998-11-26)
Cell cycle checkpoints are gating mechanisms that govern cell cycle progression in the presence of DNA damage and incomplete DNA replication. The Schizosaccharomyces pombe Rad1 protein is an essential component of cell cycle checkpoints activated by both types of genomic
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