grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
SacI is a restriction enzyme used in molecular biology applications to cleave DNA at the recognition site 5′-GAGCT/C-3′, generating DNA restriction fragments with 3′-cohesive ends.
Biochem/physiol Actions
Recognition sequence: 5′-GAGCT/C-3′
Cutting results: a 2-10-fold Sac I overdigestion of 1 μg λ Hind III DNA substrate results in 100% cutting
Heat inactivation : Inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Sac I overdigestion of 1 μg λ Hind III DNA substrate results in 100% cutting
Heat inactivation : Inactivated at 65 °C for 15 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
Other Notes
Comment: Sac I activity is inhibited by salt concentrations above 10 mM.
Supplied with 10x Restriction Enzyme Buffer SA (B7531).
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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