R5280
Anti-phospho-RanGAP1 (pSer428) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-Ran GTPase activating protein-1
产品名称
Anti-phospho-RanGAP1 (pSer428) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~60 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using HeLa cells
western blot: 1.5-3.0 μg/mL using HEK-293T cell lysate expressing human RanGAP1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer428)
Gene Information
human ... RANGAP1(5905)
Application
Anti-phospho-RanGAP1 (pSer428) antibody produced in rabbit has been used in western blotting.
Biochem/physiol Actions
Anti-phospho-RanGAP1 (pSer428) specifically recognizes human phospho-RanGAP1 (pSer428) (not yet tested in other species).
However, the SUMO-1 modification induces the interaction of RanGAP1 with the interphase nuclear pore complex (NPC) through binding to the nucleoporin Ras-related nuclear protein 1 binding protein 2 (RANBP2) and to Ubc9. RanGAP1 is phosphorylated on residues Thr409, Ser442 and Ser428. Phosphorylated RanGAP1 may aid the translocation of specific SUMO target proteins to RanBP2′s catalytic domain.
Ran GTPase Activating Protein 1 (RanGAP1) is a key regulator of Ran activity, by specifically inducing its GTPase activity. RanGAP1 is conjugated to the small ubiquitin-related modifier protein (SUMO-1). The activity of RanGAP1 is not substantially altered by SUMO-1 modification. Phosphorylation ensues before the breakdown of the nuclear envelope and is sustained during the entire mitosis process.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The gene for Ran GTPase activating protein 1 (RANGAP1) is located on the human chromosome 22q13.2.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Jomon Joseph et al.
Current biology : CB, 14(7), 611-617 (2004-04-06)
RanGAP1 is the activating protein for the Ran GTPase. Vertebrate RanGAP1 is conjugated to a small ubiquitin-like protein, SUMO-1. This modification promotes association of RanGAP1 with the interphase nuclear pore complex (NPC) through binding to the nucleoporin RanBP2, also known
Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex
Reverter D and Lima CD
Nature, 435(7042), 687-687 (2005)
ON 01910. Na inhibits growth of diffuse large B-cell lymphoma by cytoplasmic sequestration of sumoylated C-MYB/TRAF6 complex
Dai YH, et al.
Translational Research, 175(11), 129-143 (2016)
Alexandre Amlie-Wolf et al.
Nucleic acids research, 46(17), 8740-8753 (2018-08-17)
The majority of variants identified by genome-wide association studies (GWAS) reside in the noncoding genome, affecting regulatory elements including transcriptional enhancers. However, characterizing their effects requires the integration of GWAS results with context-specific regulatory activity and linkage disequilibrium annotations to
Ran GTPase-activating protein 1 is a therapeutic target in diffuse large B-cell lymphoma
Chang K C, et al.
Testing, 8(11), e79863-e79863 (2013)
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