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Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
NheI is a restriction endonuclease that is used in molecular biology to cleave DNA at the recognition site 5′-G/CTAGC-3′ to yield fragments with 5′-cohesive termini compatible with ends generated by AvrII, SpeI and XbaI.
Biochem/physiol Actions
Recognition sequence: 5′-G/CTAGC-3′
Cutting results: a 2-10-fold Nhe I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Cutting results: a 2-10-fold Nhe I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.8, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.2% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
Other Notes
Comment: High salt concentrations will inhibit activity of Nhe I.
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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