R5757
Nde II from Neisseria denitrificans NRCC 31009
buffered aqueous glycerol solution
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form
buffered aqueous glycerol solution
concentration
5,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition sequence: 5′-/GATC-3′
Ligation and recutting results: After 2-10-fold Nde II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Ligation and recutting results: After 2-10-fold Nde II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5 , 0.1 mM EDTA, 50 mM NaCl, 1 mM dithioerythritol, 50% glycerol (v/v), 0.02% polydocanol, 0.01% gelatine (v/v) at 4 °C.
Other Notes
Comment: One unit cleaves 1 μg dam methylated DNA.
Isoschizomer: Mbo I
Supplied with 10× Restriction Endonuclease Buffer SP (B6048).
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Datasheet
NdeI: a restriction endonuclease from Neisseria denitrificans which cleaves DNA at 5'-CATATG-3' sequences.
R J Watson et al.
FEBS letters, 150(1), 114-116 (1982-12-13)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Saqib H Ansari et al.
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β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a