生物来源
rabbit
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 74 kDa
种属反应性
human, rat
技术
immunoprecipitation (IP): 5 μg using 10 μg lysate of human epidermal carcinoma A431 cells
microarray: suitable
western blot: 1:8,000 using a human epidermal carcinoma A431 cell line lysate
UniProt登记号
运输
dry ice
储存温度
−20°C
基因信息
human ... RAF1(5894)
rat ... Raf1(24703)
一般描述
Anti-Raf-1 is developed in rabbits using a synthetic peptide K-TEDINACTLTTSPRLPVF corresponding to the C-terminal of Raf-1 of human origin, conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Raf-1 (c-Raf-1), a cytoplasmic serine/threonine protein kinase (73-76kD), belongs to a small family of protein kinases. Raf-1 is the prototype member of this family that include Raf-A and Raf-B and is the cellular homolog of the viral oncogene v-Raf. Raf1 is widely expressed in many tissues, whereas Raf-A and Raf-B are expressed mainly in the urogenital and brain tissues, respectively.
免疫原
synthetic peptide corresponding to amino acids 631-648 of human Raf-1.
应用
Anti-Raf-1 (631-648) antibody produced in rabbit has been used in:
- immunolabeling assays
- western blotting
- immunoprecipitation
生化/生理作用
Raf-1 (c-Raf-1) functions in the control of cell growth, differentiation and its oncogenic form can initiate malignant transformation of cells. Raf-1 has a critical role in the Ras/MAP kinase signaling pathway, integrating mitogenic signals mammalian cells from many growth factors, cytokines and oncogenes including epidermal growth factor (EGF), platelet-derived growth factor (PDGF), insulin, interleukin 2 (IL-2), IL-3, colony-stimulating factor 1 (CSF-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF). Raf-1 regulates the MAP kinase pathway by phosphorylating and activating the downstream MAP kinase kinase, MEK. c-Raf kinase is the first identified downstream effector kinase of Ras and is the activator of MEK which in turn activates ERK.
The sequence of the immunizing peptide is identical in rat Raf-1 and v-Raf from the mouse sarcoma virus. Anti-Raf-1 specifically recognises Raf-1 (74 kDa). The antibody may also detect Raf-1 degradation products at 40-60 kDa.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
MEK-1 Activates C-Raf Through a Ras-Independent Mechanism
Leicht DT, et al.
Biochimica et Biophysica Acta, 1833(5), 976-976 (2013)
Striatal GDNF administration increases tyrosine hydroxylase phosphorylation in the rat striatum and substantia nigra
Salvatore MF, et al.
Journal of Neurochemistry, 90(1), 245-254 (2004)
Takuya Hasegawa et al.
FEBS open bio, 7(8), 1154-1165 (2017-08-07)
We previously reported that microRNA-205 (miR-205) is downregulated by overexpression of the receptor tyrosine kinase ErbB2 and that ectopic transfection of miR-205 precursor decreases ErbB2 tumorigenicity in soft agar. In this study, we further analyzed the regulatory mechanisms linking ErbB2
Trihydrophobin 1 is a new negative regulator of A-Raf kinase
Liu W, et al.
The Journal of biological chemistry, 279(11), 10167-10175 (2004)
Raf Kinases: Function, Regulation and Role in Human Cancer
Leicht DT, et al.
Biochimica et Biophysica Acta, 1773(8), 1196-1196 (2007)
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