等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
5,000 units/mL
运输
wet ice
储存温度
−20°C
应用
AvaII is a restriction enxyme used in molecular biology applications to cleave the DNA recognition sequence 5′-G/G(A,T)CC-3′, generating fragments with 5′-cohesive ends.
生化/生理作用
Recognition sequence: 5′-G/G(A,T)CC-3′
Ligation and recutting results: After 2-10-fold Ava II overdigestion of 1 μg λ DNA substrate, results in 100%cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
Ligation and recutting results: After 2-10-fold Ava II overdigestion of 1 μg λ DNA substrate, results in 100%cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
外形
Solution in 10 mM Tris-HCl, pH 7.4, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA, 50% glycerol (v/v) at 4°C
其他说明
Comment: Activity of Ava II blocked by overlapping dcm methylation
Supplied with 10x Restriction Endonuclease Buffer SA (B7531).
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
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