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Merck
CN

R6004

Sigma-Aldrich

Ava II from Anabaena variabilis

buffered aqueous glycerol solution

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等级

Molecular Biology

表单

buffered aqueous glycerol solution

浓度

5,000 units/mL

运输

wet ice

储存温度

−20°C

应用

AvaII is a restriction enxyme used in molecular biology applications to cleave the DNA recognition sequence 5′-G/G(A,T)CC-3′, generating fragments with 5′-cohesive ends.

生化/生理作用

Recognition sequence: 5′-G/G(A,T)CC-3′
Ligation and recutting results: After 2-10-fold Ava II overdigestion of 1 μg λ DNA substrate, results in 100%cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.

外形

Solution in 10 mM Tris-HCl, pH 7.4, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA, 50% glycerol (v/v) at 4°C

其他说明

Comment: Activity of Ava II blocked by overlapping dcm methylation
Supplied with 10x Restriction Endonuclease Buffer SA (B7531).

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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M Nannipieri et al.
Journal of the American Society of Nephrology : JASN, 10(7), 1530-1541 (1999-07-15)
Approximately 30% of diabetic patients develop nephropathy, the appearance of which is partially under genetic control. Atrial natriuretic peptide (ANP) has associated physiologic effects on the kidney. This study was conducted to examine the relationship between a newly identified and
K Murray et al.
The Biochemical journal, 159(2), 317-322 (1976-11-01)
Two endonucleases, AvaI and AvaII, were isolated from Anabaena variabilis on the basis of their ability to make a limited number of breaks at specific points in bacteriophage lambda DNA. Neither enzyme has cofactor requirements beyond Mg2+. Endonuclease AvaI makes
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Gajendradhar R Dwivedi et al.
Nucleic acids research, 41(5), 3274-3288 (2013-01-29)
Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism

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