等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
应用
BglII is a DNA restriction enzyme used for molecular biology methods to cut the recognition sequence 5′-A/GATCT-3′,generating DNA fragments with 5′-cohesive ends.
生化/生理作用
Recognition sequence: 5′-A/GATCT-3′
Cutting results: a 2-10-fold Bgl II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat Inactivation: This enzyme cannot be heat inactivated.
Cutting results: a 2-10-fold Bgl II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat Inactivation: This enzyme cannot be heat inactivated.
外形
Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 200 mM NaCl, 10 mM 2-mercaptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4°C
其他说明
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
V Pirrotta
Nucleic acids research, 3(7), 1747-1760 (1976-07-01)
The sites of action of the restriction enzyme Bgl II on lambda DNA are mapped. This enzyme recognises the sequence 5' ...AGATCT...3' and makes staggered cuts producing sticky ends. In lambda DNA, the second A in this sequence is methylated
P Manivasakam et al.
Nucleic acids research, 29(23), 4826-4833 (2001-12-01)
Mammalian cells repair DNA double-strand breaks by illegitimate end-joining or by homologous recombination. We investigated the effects of restriction enzymes on illegitimate and homologous DNA integration in mammalian cells. A plasmid containing the neo(R) expression cassette, which confers G418 resistance
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
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