grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
BglI is a restriction enzymes used in molecular biology studies to cut DNA at the recognition sequence 5′-GCC(N)4/NGGC-3′, generating DNA fragments with 3′-cohesive termini.
Biochem/physiol Actions
Recognition sequence: 5′-GCC(N)4/NGGC-3′
Ligation and recutting results: After 2-10-fold Bgl I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivation is achieved at 65 °C for 15 minutes.
Ligation and recutting results: After 2-10-fold Bgl I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivation is achieved at 65 °C for 15 minutes.
Physical form
Solution in 50 mM Tris-HCl, pH 7.0, 1 mM EDTA, 200 mM NaCl, 7 mM 2-Mercaptoethanol, 50% glycerol (v/v) at 4°C
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B 3657).
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Instructions
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