grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition sequence: 5′-GCATG/C-3′
Cutting results: A 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Cutting results: A 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl , pH 8.2, 1 mM EDTA, 350 mM NaCl, 7 mM 2-mercaptoethanol, 0.1 mM PMSF, 50% glycerol (v/v), 0.2% Triton X-100 (v/v), at 4 °C
Other Notes
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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