等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
生化/生理作用
Recognition sequence: 5′-GCATG/C-3′
Cutting results: A 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Cutting results: A 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
外形
Solution in 20 mM Tris-HCl , pH 8.2, 1 mM EDTA, 350 mM NaCl, 7 mM 2-mercaptoethanol, 0.1 mM PMSF, 50% glycerol (v/v), 0.2% Triton X-100 (v/v), at 4 °C
其他说明
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Characterization of a site-specific restriction endonuclease Sph l from Streptomyces phaeochromogenes.
Fuchs, L.Y., et al.
Gene, 10, 391-391 (1980)
C Kessler et al.
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Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against
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Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
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