grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition sequence: 5′-GCATG/C-3′
Cutting results: A 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Cutting results: A 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl , pH 8.2, 1 mM EDTA, 350 mM NaCl, 7 mM 2-mercaptoethanol, 0.1 mM PMSF, 50% glycerol (v/v), 0.2% Triton X-100 (v/v), at 4 °C
Other Notes
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Characterization of a site-specific restriction endonuclease Sph l from Streptomyces phaeochromogenes.
Fuchs, L.Y., et al.
Gene, 10, 391-391 (1980)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
D S Jansson et al.
British poultry science, 53(6), 790-799 (2013-02-13)
1. Vancomycin-resistant Enterococcus faecium (VRE(fm)) has recently spread among Swedish broiler farms. The objectives were to investigate VRE(fm) persistence within barns between flocks, and to determine whether day-old chicks, feed or forklift trucks used for loading crates could be identified