form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition sequence: 5′-CC/NGG-3′
Ligation and recutting results: After 2-10-fold ScrF I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
Ligation and recutting results: After 2-10-fold ScrF I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 7.5 , 0.1 mM EDTA, 1 mM dithiothreitol, 50 mM KCl, 50% glycerol (v/v), 200 μg/ml BSA at 4 °C
Other Notes
Comment: ScrF I will only partially cleave DNA isolated from E. coli strains that have the dcm methylase (dcm+ strains).
Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
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G F Fitzgerlad et al.
Nucleic acids research, 10(24), 8171-8179 (1982-12-20)
A novel sequence-specific endonuclease has been isolated from Streptococcus cremoris F. ScrFI recognises the sequence: (formula; see text) and cleaves as indicated by the arrow ( ). It is the first enzyme to recognise this sequence and the first endonuclease
C Kessler et al.
Gene, 47(1), 1-153 (1986-01-01)
The properties and sources of all known restriction endonucleases and methylases are listed. The enzymes are cross-indexed (Table I), classified according to their recognition sequence homologies (Table II), and characterized within Table II by the cleavage and methylation positions, the
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Amal M El-Shehaby et al.
Scandinavian journal of clinical and laboratory investigation, 73(1), 75-81 (2012-12-04)
Left ventricular hypertrophy (LVH) is a common manifestation of cardiovascular disease and has an important prognostic value in patients with end-stage renal disease (ESRD). Vitamin D receptor (VDR) has been intensively investigated, and one of these (BsmI) already has been
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