表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
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生化/生理作用
Recognition sequence: 5′-CC/NGG-3′
Ligation and recutting results: After 2-10-fold ScrF I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
Ligation and recutting results: After 2-10-fold ScrF I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
外形
Solution in 10 mM Tris-HCl, pH 7.5 , 0.1 mM EDTA, 1 mM dithiothreitol, 50 mM KCl, 50% glycerol (v/v), 200 μg/ml BSA at 4 °C
其他说明
Comment: ScrF I will only partially cleave DNA isolated from E. coli strains that have the dcm methylase (dcm+ strains).
Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
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G F Fitzgerlad et al.
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A novel sequence-specific endonuclease has been isolated from Streptococcus cremoris F. ScrFI recognises the sequence: (formula; see text) and cleaves as indicated by the arrow ( ). It is the first enzyme to recognise this sequence and the first endonuclease
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The properties and sources of all known restriction endonucleases and methylases are listed. The enzymes are cross-indexed (Table I), classified according to their recognition sequence homologies (Table II), and characterized within Table II by the cleavage and methylation positions, the
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