等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
应用
SfiI is a DNA restriction enzyme that is used in molecular biological methods to cleave DNA at the recognition site 5′-GGCC(N)4/NGGCC-3′ to generate fragments with 3′-cohesive termini.
生化/生理作用
Recognition sequence: 5′-GGCC(N)4/NGGCC-3′
Cutting results: a 2-10-fold Sfi I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Cutting results: a 2-10-fold Sfi I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
外形
Solution in 10 mM Tris-HCl, pH 7.4, 0.1 mM EDTA, 5 mM MgCl2, 300 mM KCl, 7 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v), 500 μg/ml BSA at 25 °C
其他说明
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
J M Johnson et al.
Applied and environmental microbiology, 61(7), 2806-2808 (1995-07-01)
Food and patient isolates from an Escherichia coli O157:H7 outbreak associated with undercooked ground beef were characterized by pulsed-field gel electrophoresis and Shiga-like toxin genotype. Pulsed-field gel electrophoresis confirmed the epidemiologically implicated source of the two-state outbreak and differentiated between
B Q Qiang et al.
Nucleic acids research, 12(11), 4507-4516 (1984-06-11)
A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus . This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN / NGGCC , symmetrically
M Van Looveren et al.
Journal of clinical microbiology, 36(10), 2828-2834 (1998-09-17)
In Belgium an increase in the incidence of meningococcal disease has been noted since the early 1990s. Four hundred twenty clinical strains isolated during the period from 1990 to 1995, along with a set of 30 European reference strains, and
C Kessler et al.
Gene, 47(1), 1-153 (1986-01-01)
The properties and sources of all known restriction endonucleases and methylases are listed. The enzymes are cross-indexed (Table I), classified according to their recognition sequence homologies (Table II), and characterized within Table II by the cleavage and methylation positions, the
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
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