R9376
AMV-Reverse Transcriptase from avian myeloblastosis virus
别名:
RNA-directed DNA polymerase, Revertase
form
solution
concentration
10,000-100,000 units/mL
shipped in
dry ice
storage temp.
−70°C
Application
AMV reverse transcriptase synthesizes DNA complementary (cDNA) to RNA templates. A DNA primer complementary to the RNA template and a divalent cation, either Mg or Mn, are required for initiation of transcription. This enzyme is commonly used to make cDNAs from mRNA for eventual cloning or for use as probes.
Other Notes
One unit incorporates 1 nmol of dTTP into TCA precipitable material in 10 min at 37 °C using polyadenylic acid as template and oligo(dT)12-18 as a primer.
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
法规信息
新产品
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Heng Zhang et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(20), 8290-8295 (2013-05-03)
DNA methylation is an important epigenetic mark in many eukaryotic organisms. De novo DNA methylation in plants can be achieved by the RNA-directed DNA methylation (RdDM) pathway, where the plant-specific DNA-dependent RNA polymerase IV (Pol IV) transcribes target sequences to
Oumar Samassekou et al.
Journal of hematology & oncology, 6, 26-26 (2013-04-04)
The predominant mechanism by which human tumors maintain telomere length is via telomerase. In ~10% of tumor samples, however, telomere length is conserved, despite no detectable telomerase activity, in part through activation of the alternative lengthening of telomeres (ALT) pathway.
Ausubel, F., et al.
Short Protocols in Molecular Biology, 14-14 (1995)
TERT hypermethylation: biomarker in paediatric brain tumours.
Scott J Diede
The Lancet. Oncology, 14(6), 447-448 (2013-04-20)
Jiansen Jiang et al.
Nature, 496(7444), 187-192 (2013-04-05)
Telomerase adds telomeric repeats to chromosome ends using an internal RNA template and a specialized telomerase reverse transcriptase (TERT), thereby maintaining genome integrity. Little is known about the physical relationships among protein and RNA subunits within a biologically functional holoenzyme.
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