R9376
AMV-Reverse Transcriptase from avian myeloblastosis virus
别名:
RNA-directed DNA polymerase, Revertase
表单
solution
浓度
10,000-100,000 units/mL
运输
dry ice
储存温度
−70°C
应用
AMV reverse transcriptase synthesizes DNA complementary (cDNA) to RNA templates. A DNA primer complementary to the RNA template and a divalent cation, either Mg or Mn, are required for initiation of transcription. This enzyme is commonly used to make cDNAs from mRNA for eventual cloning or for use as probes.
其他说明
One unit incorporates 1 nmol of dTTP into TCA precipitable material in 10 min at 37 °C using polyadenylic acid as template and oligo(dT)12-18 as a primer.
法律信息
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
法规信息
新产品
此项目有
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Short Protocols in Molecular Biology, 14-14 (1995)
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