species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type cell culture supernatant(s)
sample type plasma
sample type serum
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 24.58-6000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TNF(7124)
General description
The Human TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-α in serum, plasma, cell culture supernatants and urine.
Immunogen
Recombinant Human TNF-α
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Tumor Necrosis Factor α ELISA Kit has been used to measure the concentration of TNF-α in pre-adipocyte growth medium, blood sample and in human bronchial epithelial cell (BEAS 2B) culture supernatantand normal human keratinocytes (NHK) cell culture supernatant.
Biochem/physiol Actions
Tumor necrosis factor- α (TNF-α) helps in regulating immune response and it acts as a proinflammatory cytokine. TNF-α is implicated in the immune pathogenesis of various autoimmune, inflammatory diseases and hepatitis B virus (HBV) infection. Overexpression of the protein has been observed in serum from oral lichen planus (OLP) patients. Mutation in the gene promotor increases the risk of susceptibility to diffuse large B-cell lymphoma (DLBCL) and marginal zone lymphoma.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
存储类别
8A - Combustible corrosive hazardous materials
法规信息
新产品
此项目有
Association between TNF alpha-308 G/A polymorphism and oral lichen planus (OLP): a meta-analysis
Zhou Yand Vieira AR
Journal of Applied Oral Science : Revista FOB, 26 (2018)
Tanshinone II is a potent candidate for treatment of lipopolysaccharide-induced acute lung injury in rat model
Li L, et al.
Oncology Letters, 15(2), 2550-2554 (2018)
Human leukocyte antigen class I and II alleles in non-Hodgkin lymphoma etiology
Wang SS, et al.
Blood, 115(23), 4820-4823 (2010)
Luca Fusaro et al.
Frontiers in bioengineering and biotechnology, 8, 281-281 (2020-04-23)
Cardiovascular diseases represent the leading cause of death in developed countries. Modern surgical methods show poor efficiency in the substitution of small-diameter arteries (<6 mm). Due to the difference in mechanical properties between the native artery and the substitute, the
Ling Li et al.
Oncology letters, 15(2), 2550-2554 (2018-02-13)
The present study aimed to investigate the effect of tanshinone II, isolated from Salvia miltiorrhiza Bunge, on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats. Male Sprague-Dawley rats were divided into three groups: Control, LPS and tanshinone II. Animals in
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