biological source
Staphylococcus aureus
assay
95% (SDS-PAGE)
form
lyophilized powder
specific activity
500-1000 units/mg protein
storage temp.
−20°C
Application
CleanCut Protease can be used to cleave fusion proteins with the specific sequence LVPWELQ↓-E(1).
CleanCut Protease has been used in a study to assess enzymatic cleaning of inorganic ultrafiltration membranes used for whey protein fractionation. Clean cut protease has also been used in a study to investigate immobilized residue-specific endoproteinases for protein sequencing.
Physical form
Supplied as a lyophilized powder containing NaCl, KCl, and sodium and potassium phosphate
Other Notes
One unit will cleave >90% of 100 μg of cleavage control protein in 100 mM Tris-HCl, pH 8.0, in a total volume of 100 μL at 20 °C in 16 hours.
存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Enzymatic cleaning of inorganic ultrafiltration membranes used for whey protein fractionation
Arguello, M.
Journal of Membrane Science , 216, 121-134 (2003)
Biochemical and structural characterization of SpID protease from Staphylococcus aureus
Zdzalik M. et al.
PLoS ONE, 8(10), e76812-e76812 (2013)
P B Conrad et al.
Biotechnology and bioengineering, 57(6), 631-641 (1999-04-01)
Recovery of an aqueous bioconversion product from complex, two-phase Pseudomonas putida broths containing 20% (v/v) soybean oil presents a significant challenge for downstream processing. Although not used before in multiple-phase separation for complex biotech products, crossflow filtration employing ceramic filters
Immobilized residue-specific endoproteinases for protein sequencing
Ronnenberg, J., et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 656, 169-177 (1994)
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