物料
polystyrene
描述
Protein purification of greater than 90%
flat bottom
保质期
Once opened, product to be used within one week.
容量
≥4 μg/well binding capacity
储存温度
2-8°C
2-8°C
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一般描述
HIS-Select HC 96 well plats are coated with a high-density nickel chelate matrix. This enables high capacity affinity binding of recombinant fusion protein with histidine tags.
HIS-Select High Capacity (HC) Coated Plates are coated with a proprietary high-density nickel chelate matrix. This high capacity coating provides a greater per-well binding capacity than any other commercial histidine binding plate and can be used in a variety of applications including in-well protein quantification, high throughput purification, and post-capture analysis such as MALDI. These plates are also pre-treated to reduce non-specific binding. Manufactured under ISO 9002 in Sigma′s GMP facility.
应用
Plates suitable for use with direct quantification (BCA, Bradford or Coomassie blue), western blotting,affinity purification and mass spectrometry.
法律信息
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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Amy F MacRae et al.
Journal of biotechnology, 112(3), 247-253 (2004-08-18)
Although the expression of histidine (His)-tagged proteins in bacteria is routine, few His-tagged proteins have been expressed in plants, and no His-tagged proteins from bacterial pathogens have been expressed in plants, to our knowledge. Here, we demonstrate expression of the
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