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Merck
CN

S6657

Superdex®

Superdex®75 Prep Grade

别名:

Superdex树脂

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UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
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form

suspension (20% aqueous ethanol)

technique(s)

protein array: suitable

matrix

cross-linked agarose

particle size

24-44 μm

pore size

3-70 kDa MW range (proteins), 500-30,000 Da MW range (dextrans)

operating pH range

1-14(short term), 3-12(long term)

storage temp.

2-8°C

Quality Level

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General description

将葡聚糖凝胶基质的高选择性与交联琼脂糖的化学和物理稳定性相结合。

Application

Superdex® 可用于蛋白质层析、凝胶过滤层析、凝胶过滤介质、树脂和分离介质。Superdex® 已被用于从食用菌 猴头菇 中纯化和表征一种新型漆酶。Superdex ® 还被用于 紫荆 种子作为潜在人类肝细胞癌抑制剂的纯化和机理研究。
针对尺寸排阻色谱法的高分辨率制备分离进行了优化。列出了球状蛋白和葡聚糖的推荐分馏范围。

Legal Information

Superdex is a registered trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

存储类别

3 - Flammable liquids

wgk

WGK 3

flash_point_f

104.0 °F

flash_point_c

40 °C

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Journal of chromatography. B, Biomedical sciences and applications, 737(1-2), 143-150 (2000-02-19)
A kinesin gene has been cloned by RT-PCR (reverse transcription polymerase chain reaction) from Trypanosoma brucei and the corresponding protein overexpressed as a recombinant His-tag (histidine-tag) kinesin in E. coli in order to study its biochemical properties and to determine
Xindu Geng et al.
Journal of biotechnology, 113(1-3), 137-149 (2004-09-24)
A new technology for renaturation with simultaneous purification of the recombinant human interferon-gamma (rhIFN-gamma) in downstream of biotechnology is presented. The strategies to develop the new technology in industry scale were suggested. Based on chemical equilibrium and molecular interactions, the
Cornelia Steinhauer et al.
Proteomics, 6(15), 4227-4234 (2006-07-11)
Antibody-based microarray is a novel technology with great promise in biomedicine that will provide unique means to perform global proteome analysis. In the process of designing the high-density antibody microarrays required, several critical key issues have been identified that remain
Toshiro Matsunaga et al.
Plant physiology, 134(1), 339-351 (2003-12-13)
Borate ester cross-linking of the cell wall pectic polysaccharide rhamnogalacturonan II (RG-II) is required for the growth and development of angiosperms and gymnosperms. Here, we report that the amounts of borate cross-linked RG-II present in the sporophyte primary walls of
M Belew et al.
Journal of chromatography. A, 679(1), 67-83 (1994-09-09)
Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), produced as inclusion bodies in genetically transformed Escherichia coli cells was purified to homogeneity by a three-step chromatographic procedure involving hydrophobic interaction, ion exchange and gel filtration. Each purification step is reproducible and well

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