生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
34kDa
种属反应性
zebrafish
浓度
1mg/mL
技术
immunohistochemistry: suitable
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
基因信息
zebrafish ... spi1b(30117)
一般描述
PU.1 is a transcription factor that belongs to the erythroblast transformation specific (ETS) family. This protein is highly expressed in hematopoietic cells like mast cells, B lymphocytes, neutrophils, early erythroblasts, and macrophages. PU.1 protein consists of a highly conserved DNA binding domain, a glutamine-rich domain, acidic residues towards the N-terminal, and a central proline-glutamic acid-serine-threonine (PEST) domain.
免疫原
Recombinant protein encompassing a sequence within the center region of zebrafish PU.1/Spi1.
应用
Suggested starting dilutions are as follows: IHC-Wm: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
生化/生理作用
PU.1 transcription factor facilitates the regulation of myeloid differentiation. This transcription factor is also involved in regulating several other myeloid genes such as macrophage colony-stimulating factor (M-CSF) receptor, CD11 antigen-like family member B (CD11B), granulocyte colony-stimulating factor (G-CSF) receptor, and granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor. Dysregulation of the PU.1 gene results in an increase in erythroblast proliferation, inhibits erythroid differentiation and leads to erythroleukemia.
特点和优势
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
外形
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
其他说明
Purification: Purified by antigen-affinity chromatography
免责声明
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
P Zhang et al.
Blood, 96(8), 2641-2648 (2000-10-07)
The lineage-specific transcription factors GATA-1 and PU.1 can physically interact to inhibit each other's function, but the mechanism of repression of GATA-1 function by PU.1 has not been elucidated. Both the N terminus and the C terminus of PU.1 can
Beatrice U Mueller et al.
Blood, 100(3), 998-1007 (2002-07-20)
The transcription factor PU.1 is required for normal blood cell development. PU.1 regulates the expression of a number of crucial myeloid genes, such as the macrophage colony-stimulating factor (M-CSF) receptor, the granulocyte colony-stimulating factor (G-CSF) receptor, and the granulocyte-macrophage colony-stimulating
C Nerlov et al.
Blood, 95(8), 2543-2551 (2001-02-07)
The GATA-1 transcription factor is capable of suppressing the myeloid gene expression program when ectopically expressed in myeloid cells. We examined the ability of GATA-1 to repress the expression and function of the PU.1 transcription factor, a central regulator of
S R McKercher et al.
The EMBO journal, 15(20), 5647-5658 (1996-10-15)
PU.1 is a member of the ets family of transcription factors and is expressed exclusively in cells of the hematopoietic lineage. Mice homozygous for a disruption in the PU.1 DNA binding domain are born alive but die of severe septicemia
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