SAB4200044
Anti-dUTPase antibody, Rat monoclonal
clone HUTP 3E6, purified from hybridoma cell culture
别名:
Anti-DUT, Anti-Deoxyuridine triphosphatase, Anti-Deoxyuridine triphosphate nucleotidohydrolase, Anti-dUTP pyrophosphatase, Monoclonal Anti-dUTPase antibody produced in rat
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NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
HUTP 3E6, monoclonal
Application:
ICC, IP, WB
Citations:
11
biological source
rat
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HUTP 3E6, monoclonal
form
buffered aqueous solution
mol wt
antigen ~22 kDa
species reactivity
mouse, marmoset, human
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.25-0.5 μg/mL using using Raji cell extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DUT(1854)
General description
Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) maintains the genomic integrity of cells. This enzyme is a symmetrical trimer with 152 amino acids.
Monoclonal Anti-dUTPase (rat IgG2a isotype) is derived from the hybridoma HUTP 3E6 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from a rat immunized with protein. The DUT gene is mapped on the human chromosome at 15q21.1.
Immunogen
human dUTPase protein.
Application
Monoclonal Anti-dUTPase antibody produced in rat may be used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) hydrolyses dUTP to dUMP and pyrophosphate and promotes nucleotide metabolism. Epstein-Barr virus (EBV) encoded dUTP plays a crucial role in pathophysiology of EBV related diseases, by regulating cellular microenvironment. It is used as a target for anticancer chemotherapy.
Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.
Monoclonal Anti-dUTPase recognizes human, mouse and marmoset dUTPase.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Kinetic properties and specificity of trimeric Plasmodium falciparum and human dUTPases
Quesada-Soriano I, et al.
Biochimie, 92(2), 178-186 (2010)
Crystal structure of a dUTPase
Cedergren-Zeppezauer, et al.
Nature, 355(6362), 740-740 (1992)
Hideki Makishima et al.
Blood, 117(21), e198-e206 (2011-02-25)
Progression of chronic myelogenous leukemia (CML) to accelerated (AP) and blast phase (BP) is because of secondary molecular events, as well as additional cytogenetic abnormalities. On the basis of the detection of JAK2, CBL, CBLB, TET2, ASXL1, and IDH1/2 mutations
J Fleischmann et al.
International journal of cancer, 84(6), 614-617 (1999-11-24)
We report the generation of 2 monoclonal antibodies (MAbs), 2B12 and 3E6, suitable for the detection of human dUTPase in routinely processed paraffin sections by immunohistochemistry. Using these MAbs, we observed nuclear expression of dUTPase in the proliferation zones of
Epstein-Barr virus encoded dUTPase containing exosomes modulate innate and adaptive immune responses in human dendritic cells and peripheral blood mononuclear cells
Ariza M, et al.
PLoS ONE, 8(7), e69827-e69827 (2013)
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